Mutations of Chinese Hamster Somatic Cells from 2-Deoxygalactose Sensitivity to Resistance |
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| Authors: | Jean-Michel Claverie Aristide Comlan De Souza and Jean-Paul Thirion |
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| Institution: | Départment de Microbiologie, Centre Hospitalier Universitaire, Faculte de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada JIH 5N4 |
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| Abstract: | In Chinese hamster somatic cells, the spontaneous change of phenotype from 2-deoxygalactose sensitivity to resistance was studied using fluctuation test experiments à la Luria and Delbrück (1943) for four Chinese hamster cell strains derived from V79. The results are consistent with true mutational events. The mutation rates are in the range of 1 to 3.5 X 10(-5) per cell per generation. The relationship between the 2-deoxyglactose resistance and the galactokinase markers is discussed. |
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