Isolation and characterization of two entomopathogenic fungi attackingDiaphorina citri (Homoptera, Psylloidea) in Indonesia |
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| Authors: | Siti Subandiyah Naruo Nikoh Hiroki Sato Franciscus Wagiman Shinji Tsuyumu Takema Fukatsu |
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| Affiliation: | (1) Department of Entomology and Plant Pathology, Faculty of Agriculture, Gadjah Mada University, 55281 Yogyakarta, Indonesia;(2) Agency of Industrial Science and Technology, National Institute of Bioscience and Human-Technology, 305-8566 Tsukuba, Ibaraki, Japan;(3) Bio-Oriented Technology Research Advancement Institution, 331-8537 Omiya, Saitama, Japan;(4) Insect Pathology Laboratory, Forestry and Forest Products Research Institute, Tsukuba Norin Danchi-nai, P.O. Box 16, 305-0903 Ibaraki, Japan;(5) Faculty of Agriculture, Shizuoka University, 422-8017 Shizuoka, Japan |
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| Abstract: | In an attempt to suppress the propagation of citrus greening disease in Indonesia, we explored pathogens ofDiaphorina citri which vectors the disease. At two orange orchards, manyD. citri adults were found to be dead and covered with fungal mycelia. Two fungi,Paecilomyces fumosoroseus andHirsutella citriformis, were consistently isolated from the infected insects. Molecular phylogeny of their 18S rDNA sequences showed that they belong to the ascomycetous clade of the Clavicipitales/Hypocreales, which embraces many entomopathogenic fungi. When healthy adults ofD. citri were inoculated with conidia of theP. fumosoroseus, the insects died within 6 d. |
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| Keywords: | biological control citrus greening disease Diaphorina citri Hirsutella citriformis Paecilomyces fumosoroseus |
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