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Artificial diet and rearing methods for Thanasimus dubius (Coleoptera: Cleridae), a predator of bark beetles (Coleoptera: Scolytidae)
Authors:John D. Reeve   M. Guadalupe Rojas  Juan A. Morales-Ramos
Affiliation:a Department of Zoology, Southern Illinois University, Carbondale, IL 62901, USA;b Formosan Subterranean Termite Research Unit, Southern Regional Research Center, USDA-ARS, New Orleans, LA 70124, USA
Abstract:Clerid beetles are common natural enemies of bark beetles, and could potentially be used as biological control agents if they could be reared in sufficient numbers. We developed an artificial diet devoid of insect components for rearing Thanasimus dubius (Fabricius), a clerid that attacks several economically important bark beetles in eastern North America. We reared larvae of this predator using the artificial diet, and then used either natural or factitious prey to feed the adults so produced. Two different methods of presenting the diet were also examined. We then compared the performance of T. dubius reared on the artificial diet with newly-emerged wild individuals collected from the field. Our results suggest that adult predators reared on the diet are near in quality to wild ones, and high R0 values can be obtained. No difference in prey preference was found between wild and diet-reared individuals after five generations in the laboratory. Sufficient numbers of predators could be generated using these techniques to permit limited field trials of augmentative biological control.
Keywords:Artificial diet   Biological control   Thanasimus dubius   Dendroctonus frontalis   Ips grandicollis   Callosobruchus maculatus
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