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Expression of ferritin-like protein in Listeria monocytogenes after cold and freezing stress |
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| Authors: | Hanene Miladi Abdelaziz Soukri Amina Bakhrouf Emna Ammar |
| Institution: | 1. Laboratoire d??Analyses, Traitement et Valorisation des Polluants de l??Environnement et des Produits, Facult?? de Pharmacie, rue Avicenne, 5000, Monastir, Tunisia 3. Unit?? de Recherche Gestion des Environnements Urbains et C?tiers?CLARSEN, Ecole Nationale d??Ing??nieurs de Sfax (Tunisie), B.P. ?W?, 3038, Sfax, Tunisia 2. Laboratoire de Physiologie et de G??n??tique Mol??culaire (PGM), Facult?? des Sciences, Ain-Chock,, BP 5366, Maarif Casablanca, Morocco |
| Abstract: | The cold shock protein family consists of the transfer of the foodborne pathogen Listeria monocytogenes from 37 to 4 and ?20?°C and was characterized by the sharp induction of a low molecular mass protein. This major cold shock protein ferritin-like protein (Flp) has an important role in regulation of various microbial physiological processes. Flp have a molecular mass of about 18?kDa, as observed on SDS?CPAGE. The purification procedure including ammonium sulfate fractionation was used. Monospecific polyclonal antibodies raised in rabbits against the purified new Flp immunostained a single 18-kDa Flp band in extracts from different cytoplasmic proteins blotted onto nitrocellulose. A 411-bp cDNA fragment that corresponds to an internal region of an flp gene was obtained by RT-PCR. Our result indicated a surexpression of major cold shock protein and an important increase in flp mRNA amount after a downshift temperature especially at ?20?°C. |
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