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Expression of neutralizing epitope of porcine epidemic diarrhea virus in potato plants |
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| Authors: | Young-Sook?Kim Tae-Jin?Kang Yong-Suk?Jang |
| Institution: | (1) Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University, South Korea;(2) Institute of Basic Science, Chonbuk National University, South Korea;(3) Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University, Jeonju, Chonbuk, 561–756, South Korea |
| Abstract: | Transgenic plants expressing recombinant proteins from pathogenic microorganisms provide an inexpensive edible vaccine for induction of local immunity. A neutralizing epitope of porcine epidemic diarrhea virus (PEDV) gene containing SEKDEL was expressed in potato using Agrobacterium-mediated transformation system. Putative transgenic plants were regenerated, and genomic PCR confirmed the presence of PEDV epitope gene in the potato plants. Based on the ELISA results, epitope of PEDV protein made up approximately 0.1% of the total soluble tuber protein. |
| Keywords: | neutralizing epitope plant expression porcine epidemic porcine epidemic diarrhea virus transgenic potato |
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