Light and dark rate-determining steps in electron transport reactions in spinach chloroplasts

Effects of various factors, such as uncouplers, inhibitors andinhibitory treatments of chloroplasts, on light-dependent (K_L)and -independent (K_D) parameters estimated from the light intensity-activityrelationship, were studied. In the Hill-reaction with ferricyanide or methyl viologen aselectron acceptor, a reagent or treatment affecting electrontransport on one side of system II, which included the rate-limitingstep for the entire electron transport, affected only K_D. Incontrast, a change in the rate of electron transfer on the otherside of system II affected only K_L. We inferred that K_D representsthe rate of the dark rate-limiting step at infinite light intensity.On the other hand, K_L is concerned to the quantum yield of theprimary reaction, as well as to the rate constant of the reactionon the side of system II opposite to that of the rate-limitingstep at infinite light intensity. Effects of inhibitors and treatments on the reaction parameterschanged markedly depending on the pH of the reaction medium.However, the contrasting effects of inhibitors affecting theopposite sides of system II were consistently observed in definedlevels of pH. This was also the case in the photoreduction ofmethyl viologen with the ascorbate-DPIP couple as electron donor. ¹ Present address: Department of Biology, Faculty of Science,Toho University, Narashino, Chiba, Japan. (Received May 2, 1972; )