| 几种全长cDNA文库构建方法比较 |
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| 引用本文： | 毛新国,景蕊莲,孔秀英,赵光耀,贾继增.几种全长cDNA文库构建方法比较[J].遗传,2006,28(7):865-873. |
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| 作者姓名： | 毛新国 景蕊莲 孔秀英 赵光耀 贾继增 |
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| 作者单位： | 中国农业科学院作物科学研究所，农作物基因资源与基因改良国家重大科学工程，农业部作物种质资源与生物技术重点开放实验室,北京100081 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 全长cDNA文库是高效、大规模获得基因全序列信息的一条有效途径，尤其是对基因组庞大，近期内尚不能进行全基因组测序的生物来说，是一条开展功能基因组研究的重要途径。本文对几种全长cDNA文库的构建方法进行了概述，针对各种方法的原理及优缺点做了分析、比较，并结合本实验室的结果，重点介绍了Cap-trapper法在小麦全长cDNA文库中的应用及文库中全长基因比例判定方法。
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| 关 键 词： | 全长cDNA文库 SMART法 Oligo-cap法 Cap-jumping法 Cap-trapper法 |
| 文章编号： | 0253-9772(2006)07-0865-09 |
| 收稿时间： | 2005-07-05 |
| 修稿时间： | 2005-08-16 |
Compare of Full-length cDNA Library Construction Methods |
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| MAO Xin-Guo,JING Rui-Lian,KONG Xiu-Ying,ZHAO Guang-Yao,JIA Ji-Zeng.Compare of Full-length cDNA Library Construction Methods[J].Hereditas,2006,28(7):865-873. |
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| Authors: | MAO Xin-Guo JING Rui-Lian KONG Xiu-Ying ZHAO Guang-Yao JIA Ji-Zeng |
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| Institution: | National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm & Biotechnology/the Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | The use of full-length cDNA libraries is an effective tool to obtain complete gene information in a high-efficiency, high-throughput manner, especially in organisms with huge genomes that are not amenable to whole genome sequencing. In this review, we outlined several methods of full-length cDNA library construction and compared their advantages and disadvantages based on their respective principles. Drawing on our own experience, we described the Cap-trapper method in detail, with an emphasis on its application in wheat full-length cDNA library construction as well as the determination of the ratio of full-length cDNA in a library. |
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| Keywords: | Full-length cDNA library Cap-trapper method Capture method Oligo-capping method Cap-jumping method SMART method |
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