Apple MdABCF assists in the transportation of S‐RNase into pollen tubes |
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| Authors: | Dong Meng Zhaoyu Gu Wei Li Aide Wang Hui Yuan Qing Yang Tianzhong Li |
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| Institution: | 1. Laboratory of Fruit Cell and Molecular Breeding, College of Agronomy and Bio‐Tech, China Agricultural University, , Beijing, 100193 China;2. College of Horticulture, Shenyang Agricultural University, , Shenyang, 110866 China |
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| Abstract: | Self‐incompatibility (SI) is a reproductive isolation mechanism in flowering plants. Plants in the Solanaceae, Rosaceae and Plantaginaceae belong to the gametophytic self‐incompatibility type. S‐RNase, which is encoded by a female‐specific gene located at the S locus, degrades RNA in the pollen tube and causes SI. Recent studies have provided evidence that S‐RNase is transported non‐selectively into the pollen tube, but have not specified how this transportation is accomplished. We show here that the apple (Malus domestica) MdABCF protein, which belongs to group F of the ABC transporter family, assists in transportation of S‐RNase into the pollen tube. MdABCF is located in the pollen tube membrane and interacts with S‐RNase. S‐RNase was unable to enter the pollen tube when MdABCF was silenced by antisense oligonucleotide transfection. Our results show that MdABCF assists in transportation of either self or non‐self S‐RNase into the pollen tube. Moreover, MdABCF coordinates with the cytoskeleton to transport S‐RNase. Blockage of S‐RNase transport disrupts self‐incompatibility in this system. |
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| Keywords: | apple self‐incompatibility S‐RNase MdABCF pollen tube transportation |
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