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A METHOD OF OBTAINING AXENIC CULTURES OF TRENTEPOHLIA SPP. (CHLOROPHYTA)1 |
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| Authors: | Melin Lim Bee-Lian Ong Yeow-Chin Wee |
| Abstract: | Axenic cultures of Trentepohlia species are necessary for the study of growth and hysiological characters of the algae. We describe the use of a Sherman micromanipulator to isolate filaments from samples of T. aurea and T. odorata collected from their natural habitats. These filaments were then used as inocula for the establishment of axenic cultures. In the case of T. aurea, further treatment with lactic acid was necessary. |
| Keywords: | axenic culture Chlorophyta micromanipulator Trentepohlia aurea T odorata |