Somatostatin binding to pituitary plasma membranes. |
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| Authors: | J W Leitner R M Rifkin A Maman K E Sussman |
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| Institution: | 1. Medical Service, Denver Veterans Administration Medical Center, Denver, Colorado 80220 USA;2. the Division of Endocrinology, Department of Medicine, University of Colorado School of Medicine, Denver, Colorado 80220 USA |
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| Abstract: | A method has been developed for the study of somatostatin binding to anterior pituitary plasma membranes. When 5×10?9M 125I]Tyr1-somatostatin (SA 18 Ci/mmol) was incubated with isolated pituitary plasma membranes (protein = 100 μg), 13.6% of total radioactivity was bound excluding nonspecific binding. The Scatchard plot could be resolved into two distinct components and analyzed to yield: K1diss = 3.3×10?8M and K2diss = 7.7×10?6M. This binding was shown to be specific for somatostatin. |
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