A new enzymatic route to the synthesis of 12-ketoursodeoxycholic acid |
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| Authors: | Roberto Bovara Giacomo Carrea Sergio Riva Francesco Secundo |
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| Institution: | (1) Istituto di Chimica degli Ormoni, C.N.R., Via Mario Bianco 9, 20131 Milano, Italy |
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| Abstract: | Summary Cholic acid (3 ,7,12-trihydroxy-5 -cholanoic acid) was completely and selectively transformed into 12-ketoursodeoxycholic acid (3,7-dihydroxy-12-oxo-5-cholanoic acid) by means of two consecutive enzymatic steps catalyzed, the first, by 7- and 12-hydroxysteroid dehydrogenase and, the second, by 7-hydroxysteroid dehydrogenase. Coenzyme regeneration was carried out with -ketoglutarate-glutamate dehydrogenase and glucose-glucose dehydrogenase, respectively. |
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