Synthetic oligonucleotides for biomedical applications. |
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| Authors: | H Seliger A Fr?hlich G Gr?ger B Krist M Montenarh H R?sch R R?sch F R Ortigao |
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| Affiliation: | Universit?t Ulm, Sektion Polymere, FRG. |
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| Abstract: | ![]()
New developments of oligonucleotides for biomedical applications are surveyed. Diagnostic probes were conveniently labelled by enzymatic immunogenic tailing with 5-bromo-deoxyuridine triphosphate. "Antisense" oligonucleotides of potential therapeutic value were stabilized against nucleolytic decay by inversion of terminal internucleotidic linkages. Introduction of 2'-deoxy-2'-fluoro-nucleotide units enhances duplex stability and conveys resistance to ribonucleases. |
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