Cell-free translation of exogenous mRNA in extracts from dry pea primary axes |
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Authors: | Willy J Peumans Albert R Carlier Jeanine Schreurs |
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Institution: | (1) Laboratorium voor Plantenbiochemie, Katholieke Universiteit Leuven, Vaartstraat 24, B-3000 Leuven, Belgium |
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Abstract: | Extracts from the primary axes of dry pea (Pisum sativum L.) seeds are able to perform an initiation-dependent translation of exogenous mRNA. SDS polyacrylamide gel electrophoresis of the products synthesized under direction of alfalfa mosaic virus RNA (AMV-RNA) and tobacco mosaic virus RNA (TMV-RNA) shows that the fidelity of translation in this pea system is at least as high as in a wheat embryo cell-free protein synthesizing system. The endogenous messengers are also efficiently translated in extracts from the primary axes of pea seeds. The direct translation of these messengers in a homologous cell-free system may be of interest for a study of the products coded for by the long-lived messengers present in this plant.Abbreviations HEPES
N-2-hydroxyethylpiperazine-N-ethanesulfonic acid
- SDS
sodium dodecyl sulphate
- mRNP
messenger ribonucleoprotein
- AMV-RNA
alfalfa mosaic virus RNA
- TMV-RNA
tobacco mosaic virus RNA
- ATA
aurin tricarboxylic acid
- TCA
trichloroacetic acid
- S.A.
specific activity |
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Keywords: | mRNA Protein synthesis Pisum Seeds (translation) Translation |
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