磷酸肌酸对新生鼠缺氧缺血性脑损伤线粒体功能的影响

目的:探讨外源性磷酸肌酸(PCr)在缺氧缺血性脑损伤中对线粒体功能的影响。方法:将96只七日龄Wistar新生鼠随机分为四组,分别为A假手术组、B生理盐水对照组、C磷酸肌酸小剂量干预组、D磷酸肌酸大剂量干预组,每组24只。除假手术组外其余三组动物制成HIBD模型,并在术前1h分别给予生理盐水、小剂量磷酸肌酸、大剂量磷酸肌酸腹腔注射,24h后取脑,观察脑组织线粒体内三磷酸腺苷(ATP)、还原型谷胱甘肽(GSH)以及丙二醛(MDA)的含量变化。结果:外源性磷酸肌酸干预后脑组织中线粒体内ATP、GSH、MDA含量与盐水对照组相比具有差异性(P<0.05)。结论:外源性磷酸肌酸可以改善线粒体能量代谢,减轻脂质过氧化,保护脑组织。

Effect of Phosphocreatine on Mitochondrial in Brain Tissue of Neonate Rats After Hypoxia Ischemia Brain Damage(HIBD)

Objective: To observe the protective effect of phosphovreatine on cerebral mitochondrial membrane in neonate rats with hypoxic-ischomic brain damage(HIBD).Methods: 96Wistar seven day old rats were randomly assigned into four groups: contrasted group,0.9%NaCl contrasted group,PCr group 1 and PCr group 2.Every group was given shanming therapy or 0.9%NaCl or small doze PCr or large doze PCr before hypoxia-ischemia onset.To observe the contents of ATP on cerebral mitochondrial membrane in neonate rats.Result: T...