Somatic embryogenesis in <Emphasis Type="Italic">Schisandra chinensis</Emphasis> (Turcz.) Baill. |
| |
Authors: | T?D?Kim V?Ramesh?Anbazhagan Email author" target="_blank">J?I?ParkEmail author |
| |
Institution: | (1) Lab of Forest Genetic Resources, School of Forest Resources, College of Agriculture, Chungbuk National University, Cheongju, 361-763 Chungbuk, Republic of Korea |
| |
Abstract: | Summary Regeneration of plants via somatic embryogenesis was achieved from zygotic embryo explants isolated from mature seeds of Schisandra chinensis. Merkle and Sommer's medium, fortified with 2,4-dichlorophenoxyacetic acid (2,4-D; 9.04 μM) and zeatin (0.09 μM), was effective for induction of embryogenic callus. The development of a proembryogenic mass and somatic embryos occurred
on Murashige and Skoog medium (MS) free of plant growth regulators. The embryogenic callus induced on Merkle and Sommer's
medium supplemented with 2,4-D (9.04 μM) and zeatin (0.09 μM) showed development of the maximum number of somatic embryos when transferred to MS medium free of plant growth regulators.
The maximum maturation and germination of cotyledonary somatic embryos (46.3%) occurred on MS medium supplemented with 2,4-D
(0.45 μM) and N6-benzyladenine (1.11 μM). The somatic embryo-derived plants were successfully hardned, with a survival rate of approximately 67%, and established
in the field. |
| |
Keywords: | pharmaceutical value Schisandra chinensis somatic embryogenesis zygotic embryo |
本文献已被 SpringerLink 等数据库收录! |
|