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CULTURE OF THE HETEROTROPHIC DINOFLAGELLATE PROTOPERIDINIUM CRASSIPES (DINOPHYCEAE) WITH NONCELLULAR FOOD ITEMS1
Authors:Aika Yamaguchi  Takeo Horiguchi
Institution:1. Center for Advanced Marine Core Research, Kochi University, Nankoku, Kochi 783‐8502, Japan;2. Author for correspondence: e‐mail .;3. Department of Natural History Sciences, Faculty of Science, Hokkaido University, Sapporo 060‐0810, Japan
Abstract:The genus Protoperidinium is an assemblage of heterotrophic dinoflagellates, several species of which have been successfully cultured in the past using various photosynthetic algae as a food source. We succeeded in culturing Protoperidinium crassipes (Kof.) Balech on three separate occasions for periods ranging from 2 to 21 months using rice flour as a food source. In these cultures, unusual small types of cells that were never observed to actively feed sometimes appeared. We confirmed that P. crassipes in culture exhibited bioluminescence.
Keywords:bioluminescence  culture  heterotrophic dinoflagellate  pallium feeding  Protoperidinium
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