| 利用高保真酶扩增特性调整基因读码框的方法 |
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| 引用本文： | 张国广,陈亮,吴亦亮,曾雅明.利用高保真酶扩增特性调整基因读码框的方法[J].生物技术通报,2006(3):54-57. |
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| 作者姓名： | 张国广 陈亮 吴亦亮 曾雅明 |
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| 作者单位： | 厦门大学生命科学学院,细胞生物学和肿瘤细胞工程教育部重点实验室,厦门,361005 |
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| 摘 要： | 在基因工程研究中,经常要涉及到对目的基因读码框进行调整的研究。本文报道了利用高保真DNA聚合酶扩增DNA后产生平末端的特性,及限制性内切酶对一重组的原核表达载体pET28a-HA进行读码框调整的研究,测序结果显示读码框按照预期设计正确调整,表明高保真DNA聚合酶可以用于DNA 3'凹陷端的补平,在DNA 3'凹陷端平滑化时多了一个有效的选择。
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| 关 键 词： | 高保真DNA聚合酶 DNA粘端补平 基因读码框调整 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2005年12月19 |
A Convenient Method for Condon Adjustment by Using High-fidelity DNA Polymerase Amplification Property |
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| Zhang Guoguang,Chen Liang,Wu Yiliang,Zeng Yaming.A Convenient Method for Condon Adjustment by Using High-fidelity DNA Polymerase Amplification Property[J].Biotechnology Bulletin,2006(3):54-57. |
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| Authors: | Zhang Guoguang Chen Liang Wu Yiliang Zeng Yaming |
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| Institution: | The key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China |
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| Abstract: | The reading frame adjustment of target genes was often occurred in the study of gene engineering.The results of pET28-HA gene reading frame exactly adjustment showed high-fidelity DNA polymerase can be used generate blunt end from a DNA 5'-protruding end.The convenient method can be selected in the blunt of DNA 5'-protruding end. |
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| Keywords: | High-fidelity DNA polymerase Blunt of DNA 5'-protruding end Code adjustment |
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