Ultrastructural research on cell wall regeneration by cultured pollen protoplasts of Lilium longiflorum |
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Authors: | H Miki-Hirosige S Nakamura I Tanaka |
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Institution: | (1) Biological Laboratory, Kanagawa Dental College, Inaokacho 82, 238 Yokosuka, Japan;(2) Department of Biology, Yokohama City University, Kanazawa-ku, 236 Yokohama, Japan |
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Abstract: | Summary Protoplasts from pollen grains of Lilium longiflorum regenerate amorphous cellulosic cell walls in culture, during which some precursors of cellulose are polymerized, thus producing progressively harder cellulosic cell walls as the period of culture continues. It is presumed that the components of the cell wall regenerated during 1 week in culture differ from those of the intine of the pollen grain wall. The regenerated cell wall is formed by means of large smooth vesicles; in addition, numerous coated vesicles and pits aid in wall regeneration. The pollen tube that germinates from the 8-day-old cultured protoplast has numerous Golgi bodies and many vesicles which build the pollen tube wall. The tube wall has two layers just like a normal pollen tube wall. |
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Keywords: | Pollen protoplast Pollen tube Lilium longiflorum Cell wall regeneration |
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