Utilization of antipeptide antibodies as affinity ligands in immunoaffinity purification |
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| Affiliation: | 1. Service of Pharmacy, Hospital del Mar, Infectious Pathology and Antimicrobials Research Group, Institut Hospital del Mar d''Investigacions Mèdiques, Universitat Autònoma de Barcelona, Barcelona, Spain;2. Service of Infectious Diseases, Hospital del Mar, Infectious Pathology and Antimicrobials Research Group, Institut Hospital del Mar d''Investigacions Mèdiques, Universitat Autònoma de Barcelona, Barcelona, Spain;3. Service of Microbiology, Laboratori de Referència de Catalunya, Barcelona, Spain;4. Service of Pharmacy, Hospital del Mar, Universitat Autònoma de Barcelona, Barcelona, Spain;5. Service of Pharmacy, Hospital del Mar, Barcelona, Spain;6. Service of Pneumology, Hospital del Mar, Barcelona, Spain |
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| Abstract: | ![]()
Anti-peptide antibodies against the C-terminal regions of chimeric α-amylase, recombinant CD2 and insulin B-chain were obtained by using peptides corresponding to the C-terminal regions as immunogens. These anti-peptide antibodies adsorbed the native proteins, as well as the antigen peptides. The proteins were purified to high purity using the anti-peptide antibodies as affinity ligands. These ligands could discriminate the target proteins having different C-terminal regions. The adsorbed proteins were specifically eluted by the eluents containing the antigen peptides. |
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