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| 内蒙草原不同植物功能群及物种对土壤微生物组成的影响 | |
| 引用本文： | 陈颖,李肖肖,应娇妍,梁存柱,白永飞.内蒙草原不同植物功能群及物种对土壤微生物组成的影响[J].生物多样性,2012,20(1):59-65. |
| 作者姓名： | 陈颖 李肖肖 应娇妍 梁存柱 白永飞 |
| 作者单位： | 1. 内蒙古大学生命科学学院,中美生态、能源及可持续性科学内蒙古研究中心,呼和浩特010021;中国科学院植物研究所植被与环境变化国家重点实验室,北京100093 2. 中国科学院生态环境研究中心,北京,100085 3. 中国科学院植物研究所植被与环境变化国家重点实验室,北京,100093 4. 内蒙古大学生命科学学院,中美生态、能源及可持续性科学内蒙古研究中心,呼和浩特010021 |
| 基金项目： | 国家重点基础研究发展计划项目，国家自然科学基金面上项目 |
| 摘 要： | 为了分析不同植物群落组成对内蒙古典型草原土壤微生物群落组成的影响,本研究利用植物功能群剔除处理实验平台,采用荧光定量PCR(real-timePCR)和自动核糖体间隔区基因分析(automated ribosomal intergenic spacer analysis,ARISA)技术,对不同植物功能群组成的非根际土壤和常见物种的根际土壤中细菌和真菌的数量及群落结构进行了分析。结果表明,在非根际土壤中,不同植物功能群组成对细菌数量有显著影响,而对真菌数量及细菌和真菌的群落结构影响不明显;在根际土壤中,不同植物物种对细菌、真菌的数量都有显著影响。此外,聚类分析表明,不同物种的根际土中细菌和真菌的群落结构也有所不同,尤其以细菌的群落结构变化较为明显。研究结果表明不同植物物种可以通过根系影响土壤微生物群落组成。 |
| 关 键 词： | 植物多样性 根际微生物 real-time PCR ARISA |
Effects of plant functional groups and plant species on soil microbial composition in a Inner Mongolian grassland |
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| Ying Chen , Xiaoxiao Li , JiaoyanYing , Cunzhu Liang , Yongfei Bai.Effects of plant functional groups and plant species on soil microbial composition in a Inner Mongolian grassland[J].Biodiversity Science,2012,20(1):59-65. | |
| Authors: | Ying Chen Xiaoxiao Li JiaoyanYing Cunzhu Liang Yongfei Bai |
| Institution: | 1 College of Life Science,Inner Mongolia University,Sino-US Center for Conservation,Energy and Sustainability Sci-ence in Inner Mongolia,Hohhot 010021 2 State Key Laboratory of Vegetation and Environmental Change,Institute of Botany,Chinese Academy of Sciences,Bei-jing 100093 3 Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085 |
| Abstract: | In order to study the effect of plant community composition on soil microbial communities in a typical Inner Mongolian grassland,the abundance and community structure of bacteria and fungi in bulk soils under different plant functional groups and in soils from the rhizosphere of different grass species were analyzed using realtime PCR and automated ribosomal intergenic spacer analysis(ARISA) under plant functional group removal treatments.Our results indicated that changes in plant functional group affect bacterial abundance,but not fungal abundance or bacterial/fungal community structure in bulk soil.The abundance of bacteria and fungi in the rhizosphere soil varied with plant species.Cluster analysis revealed that community structure of bacteria and fungi also varied among plant species in the rhizosphere soil but not in the bulk soil.which was more evident for bacteria.Our results point to the ways in which plant species can influence soil microbial and fungal communities through the action of their roots. |
| Keywords: | plant diversity rhizosphere microorganisms real-time PCR ARISA |
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