High-level expression of soluble recombinant RNase P protein from Escherichia coli. |
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Authors: | R Rivera-Le n, C J Green, B S Vold |
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Affiliation: | R Rivera-León, C J Green, and B S Vold |
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Abstract: | We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme. |
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