C-myc、p53基因和AFP在家兔诱发性肝癌及癌旁组织中的表达

目的获得家兔二乙基亚硝胺诱发肝脏肿瘤的形态学资料,探讨Cmyc、p53基因和甲胎蛋白的表达情况。方法取5例家兔多结节肝癌的肿瘤性结节25个,瘤旁肝组织每例1块,进行一般病理组织学和超微结构观察。同时,以免疫组化方法检查Cmyc、p53基因和AFP的表达情况。结果和结论25个结节中,良性增生10个,高分化肝细胞癌11个,中分化肝细胞癌4个。家兔肝细胞癌的发生与Cmyc基因、AFP的过表达和p53基因的突变有关。参照肿瘤诱发过程中,增生性肝硬化、腺瘤样增生、高分化癌、中分化癌的变化顺序,在所检测的三种标志物中,Cmyc蛋白为最早出现的肿瘤标志物,p53基因突变相对较晚出现,AFP在恶性肿瘤结节中的表达率最高(933%)。

The Expression of C-myc,P53 and AFP in Liver Tumors and Pericarcinomatous Tissues of Rabbits Induced by Diethylnitrosamine

Objective To describe the pathology of liver tumors in rabbits induced by diethylnitrosamine and the expression of c myc, p53 genes and AFP in the tumors and pericarcinomatous tissues. Method 25 nodules in 5 rabbit livers and 5 pieces of pericarcinomatous tissues each from one liver were observed by light microscopy and electron microscopy, and the expressions of c myc gene, p53 gene and AFP were also observed in these tissues with immunohistochemical staining.Results and Conclusion There were 10 nodules of adenomatous hyperplasia, 11 of well differentiated hepatocellular carcinoma(HCC)and 4 of moderate differentiated HCC among the 25 nodules. The tumorigenesis of HCC in rabbits induced by diethylnitrosamine was related to the over expressions of c myc gene, AFP and the mutation of p53 gene. C myc over expression appeared first and p53 mutation the latest during the tumorigenesis of HCC. AFP over expression reached the top positive reaction ratio(93 3%) among the 3 detected parameters.