Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae) |
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Authors: | Qian Zhang Yan-Zhuo Yang Gui-Li Wu Dong-Yuan Zhang Jian-Quan Liu |
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Institution: | (1) MOE Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China;(2) Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, 810008, China |
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Abstract: | Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important
role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined.
In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within
and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin
capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals
from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity
ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species.
In addition, all markers have been crossly checked in the other four congeneric species. |
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Keywords: | Juniperus przewalskii Microsatellite markers Genetic diversity |
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