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Alpha-1-antitrypsin immunoreactivity in islet cells of adult human pancreas
Authors:Dr. M. B. Ray  V. J. Desmet  W. Gepts
Affiliation:(1) Departement Medische Navorsing, Academisch Ziekenhuis Sint Rafaël, Laboratorium voor Histochemie en Cytochemie, Leuven, Belgium;(2) Laboratorium voor Anatomopathologie Universitair Ziekenhuis Brugman, Brussel, Belgium;(3) Departement Medische Navorsing, Academisch Ziekenhuis Sint Rafaël, Laboratorium voor Histochemie en Cytochemie, B-3000 Leuven, Belgium
Abstract:alpha-1-antitrypsin immunoreactivity was demonstrated by immunofluorescence technique in the peripheral islet cells of all ten normal adult human pancreata examined; normal adult human liver was negative. The specificity of the reactions was confirmed by applying various control tests including absorption of the specific antisera with purified alpha-1-antitrypsin, inhibition and blocking tests and by ensuring the monospecificity of the antisera used. The findings suggest that the pancreatic islet may be an additional source of alpha-1-antitrypsin.
Keywords:Pancreas (Human)  Islet cells  Alpha-1-antitrypsin  Immunofluorescence
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