首页 | 本学科首页   官方微博 | 高级检索  
     


Isolation and characterization of a new strain of Methanothermobacter marburgensis DX01 from hot springs in China
Authors:Xia Ding  Wei-Jun Yang  Hang Min  Xiao-Tong Peng  Huai-Yang Zhou  Zhen-Mei Lu
Affiliation:1. Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea;2. Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea;3. Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA;4. Agricultural Microbiology Division, National Academy of Agricultural Science, Suwon 441-707, Republic of Korea;1. Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße 10, D-35043 Marburg, Germany;2. Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON, Canada N2L3G1;3. Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max-von-Laue-Straße 3, D-60438 Frankfurt am Main, Germany;4. Institute of Cytobiology, Philipps Universität Marburg, Robert-Koch Straße 6, 35037 Marburg, Germany;5. PRESTO, Japan Science and Technology Agency, Honcho, Kawaguchi, Saitama 332-0012, Japan
Abstract:Strain DX01, a thermophilic methanogen, was isolated from a hot spring in China. Strain DX01 grew only on H2/CO2. The DNA G + C content is 52 mol% and optimal growth temperature is 65 °C. The cell pellet is brick red. By analyzing 16S rRNA sequence, methyl-coenzyme M reductase I, gamma subunit protein sequences, we determined the DX01 strain to be closely related to the species of Methanothermobacter marburgensis. In addition, Methanothermobacter thermautotrophicus delta HT and strain DX01 had clear differences in their biochemical composition and protein expression profiles. Based on the above analysis, we propose that strain DX01 is a novel strain within thermoautotrophicus the species of M. marburgensis, namely M. marburgensis DX01. The isolation and characterization of the new M. marburgensis DX01 strain expands the known range of the Methanothermobacter genus.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号