Nucleotide sequence of the Vibrio alginolyticus glnA region |
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| Authors: | R Maharaj E Rumbak W A Jones S M Robb F T Robb D R Woods |
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| Institution: | (1) Department of Microbiology, University of Cape Town, 7700 Rondebosch, South Africa;(2) Present address: Centre of Marine Biotechnology, University of Maryland, 600 East Lombard Street, 21202 Baltimore, MD, USA |
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| Abstract: | The nucleotide sequence of a 4 kb fragment containing the Vibrio alginolyticus glnA, ntrB and ntrC genes was determined. The upstream region of the glnA gene contained tandem promoters. The upstream promoter resembled the consensus sequence for Escherichia coli  70 promoters whereas the presumptive downstream promoter showed homology with nitrogen regulated promoters. Four putative NRI binding sites were located between the tandem promoters. The ntrB gene was preceded by a single presumptive NRI binding site. The ntrC gene was located 45 base pairs downstream from the ntrB gene. The V. alginolyticus ntrB and ntrC genes were able to complement ntrB, ntrC deletions in E. coli.Abbreviations bp
base pair(s)
- CAP
catabolite-activating protein
- GS
glutamine synthetase
- kb
kilobase(s)
- ORF
open reading frame
- SD
Shine-Dalgarno |
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| Keywords: | Vibrio alginolyticus Glutamine synthetase glnA ntrB ntrC Nitrogen regulation |
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