Development and application of a loop-mediated isothermal amplification method on rapid detection of Pseudomonas aeruginosa strains |
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| Authors: | Zhao Xihong Wang Li Li Yanmei Xu Zhenbo Li Lin He Xiaowei Liu Yao Wang Jihua Yang Liansheng |
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| Institution: | (1) College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China;(2) Food Safety Key Laboratory of Guangdong Province, College of Food Science, South China Agricultural University, Guangzhou, China;(3) Guangzhou Medical College, Guangzhou, China;(4) Department of Microbial Pathogenesis, Dental School, University of Maryland-Baltimore, Baltimore, Maryland, 21201, USA;(5) Zhongshan Supervision Testing Institute of Quality & Metrology, Zhongshan, China;(6) Guangzhou Wondfo Biotechnology Company, Guangzhou, China; |
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| Abstract: | We developed and evaluated the specificity and sensitivity of a simple loop-mediated isothermal amplification (LAMP) method
for rapid detection of P. aeruginosa strains. The optimal reaction condition was found to be 65°C for 45 min, with the detection limit as 100 fg DNA/tube and
10 CFU/reaction. Application of LAMP assays were performed 426 clinical samples (including 252 P. aeruginosa and 174 non- P. aeruginosa isolates) using a rapid procedure and easy result confirmation. Sensitivity of LAMP and PCR assays was found to be 97.6%
(246/252) and 90.5% (228/252), respectively; with a 100% specificity for both assays. |
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