Interaction of mouse Pem protein and cell division cycle 37 homolog

Mouse Pem, a homeobox gene, encodes a protein consisting of 210 amino acid residues. To study the function of mouse Pem protein, we used the yeast two-hybrid system to screen the library of 7-day mouse embryo with full-length mouse Pem cDNA. Fifty-two colonies were obtained after 1.57×10^8 colonies were screened by nutrition limitation and β-galactosidase assay. Seven individual insert fragments were obtained from the library, and three of them were identified, one of which was confirmed to be the cell division cycle 37 （Cdc37） homolog gene by sequencing. The interaction between mouse Pem and Cdc37 homolog was then confirmed by glutathione S-transferase pull-down assay, and the possible interaction model was suggested.     

mPem蛋白相作用分子的筛选与鉴定

mPem是同源异型框基因,其C末端编码同源异型域。为进一步研究mPem蛋白在胚胎发育和生殖组织发育过程中的作用,利用GAL4酵母双杂交系统筛选小鼠7d胚胎cDNA文库,共获得3个与mPem蛋白相作用的分子。其中之一为Mdfic,一种新的转录调控因子。进一步的酵母双杂交和体外GST_Pulldown试验再次确认两者之间的相互作用。Mdfic与mPem蛋白之间的相互作用暗示两者有可能组成转录调控复合体,共同参与胚胎分化的调控,为两种蛋白功能的研究提供新的思路。