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This study aims to compare the resistance phenotypes conferred by various genes encoding enzymes that phosphorylate erythromycin. The mph genes were cloned into Escherichia coli AG100A susceptible to macrolides and ketolides following disruption of the AcrAB pump. An 882 bp sequence containing a premature stop codon, homologous to the three other previously described mph genes and present widely among Enterobacteriaceae, was found to confer resistance to erythromycin by phosphorylation. The mph(C) gene, as reported for mph(B), also conferred resistance to spiramycin. The mph(A) gene was unique in conferring resistance to azithromycin. The four investigated genes conferred resistance to telithromycin. 相似文献
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Roberts MC 《Molecular biotechnology》2004,28(1):47-62
Macrolides have enjoyed a resurgence as new derivatives and related compounds have come to market. These newer compounds have
become important in the treatment of community-acquired pneumoniae and nontuberculosis-Mycobacterium diseases. In this review,
the bacterial mechanisms of resistance to the macrolide, lincosamide, streptogramin, ketolide, and oxazolidinone antibiotics,
the distribution of the various acquired genes that confer resistance, as well as mutations that have been identified in clinical
and laboratory strains are examined. 相似文献
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合成酮内酯类3—脱氧—3—羰基—红霉内酯B糖多孢红霉菌M的构建 总被引:21,自引:1,他引:20
大环内酯类抗生素基因工程是近年来研究的一个新领域,迄今已合成了100多种新的聚酮类化合物。以糖多孢红霉菌A226基因组DNA为模板,用重叠PCR方法扩增出去除KR6酶域DNA的约3.2kb DNA片段,克隆于pWHM3载体,构建了同源重组质粒pWHM2201。PEG介导原生质体转化法将pWHM2201转入糖多孢红霉菌A226,并整合于染色体红霉素合成基因位点。整合体在R3M斜面上生长两代后,制备原生质体涂R3M平皿。利用PCR鉴定筛选出8株KR6敲除的突变体糖多孢红霉菌M(1-8)。ZabsPec Fab质谱鉴定,证实糖多孢红霉菌M1合成了3-脱氧-3-羰基-红霉内酯B,一种新的酮内酯类化合物。 相似文献
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