栽培花锚中抗肝炎活性成分的含量在不同生长期变化的研究

研究了花锚中去甲氧基花锚甙和花锚甙的含量随着不同生长期的变化趋势,为药材的合理栽培和采收提供科学依据。RP—HPLC法,使用VPODS C18柱,流动相为乙腈：磷酸：水(1‰),梯度洗脱程序：0～5．00min乙腈的体积分数(以下同)为15％、5．01～14．00min由15％增至25％、14．01～30．00min由25％增至40％,流速为1mL／min,柱温25C,检测波长：254nm。花锚甙和去甲氧基花锚甙、在花锚全草中的含量在不同生长期有明显变化。     

花锚的三种新(口山)酮甙

从抗肝炎植物药花锚中分得了三种新天然产(口山)酮双糖甙。经化学反应和光谱分析,化合物Ⅰ和Ⅱ分别被鉴定为1-O-[β-D-木吡喃糖-(1-6)-β-D-葡萄吡喃糖]-2,3,5,7-四甲氧基(口山)酮和1-O-[β-D-木吡喃糖-(1-6)-β-D-葡萄吡喃糖]-2,3,5-三甲氧基(口山)酮。经药理实验证明,该两成分为该植物抗肝炎的主要水溶性活性成分。化合物Ⅲ的结构初步被鉴定为1-0-[β-D-木吡喃糖-(1-6)-β-D-葡萄吡喃糖]-2,3,4,5-四甲氧基(口山)酮。     

Three New Glycosides from Halenia clliptica

Three new glycosides have been obtained from the herb Halenia elliptica D. Don (Genti- anaceae) which is used for treatment of liver diseases in Qinghai-Tibetan folk. Their structu- res have been identified as 1-O-[β-D-xylopyranosyl-(1→6)-β-D-glucopyranosyl]-2,3,5,7-terameth-oxyxanthone(Ⅰ) or as l-O-primeverosyl-2,3,5,7-tetramethoxyxanthone),1-O-[β-D-xylopyranosyl-(1-6)-β-D-glucopyranosyl]-2,3,5-trimethoxyxanthone (Ⅱ, or as 1-O-primeverosyl-2,3,5-trimeth- oxyxanthone), and 1-O-[β-D-xylopyranosyl-(1-6)-β-D-glucopyranosyl]-2,3,4,5-tetramethoxyxan- thone (Ⅲ, or as 1-O-primeverosyl-2,3,4,5-tetramethoxyxanthone) respectively, by means of che- mical and spectral methods. Ⅰ and Ⅱ are another two new antihepatitisic constituents of this species, and their biological activity of antihepatitis has been displayed by the pharmacological research. They were named haleniaside (Ⅰ) and demethoxyhaleniaside (Ⅱ).