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Screening of plasmids in non-pathogenic corynebacteria 总被引:1,自引:0,他引:1
H. Sandoval G. del Real L.M. Mateos A. Aguilar J.F. Martín 《FEMS microbiology letters》1985,27(1):93-98
Abstract A screening of plasmids in 25 nonpathogenic coryneform bacteria was carried out. 11 Strains showed at least one plasmid, ranging in size from 4.2 to 55 kb. These plasmids did not encode bacteriocin production or resistance to a number of antibiotics or to ions such as arsenite, mercury(II) and cobalt(II). A detailed study of plasmid pBL100 from Brevibacterium linens is presented. pBL100 has a size of 7.75 kb, and contains single sites for the endonucleases: Hin dIII; Pst I, Bgl II, Eco RI and Bam HI. B. linens is easily and efficiently transformed with vectors derived from pBL1 isolated from Brevibacterium lactofermentum . 相似文献
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AIMS: A wide range of lipids are present on the skin surface of human beings and bacterial lipases are known to modify them. The microflora of the underarm (axilla) is often dominated by aerobic coryneforms and whilst many require lipids for growth, they appear not to be utilized as carbon sources. The aim of this study was to investigate the adherence aerobic coryneforms to lipids present on the skin surface of the human axilla to determine whether they contribute to colonization of the skin. METHODS: Aerobic coryneforms were grown in a defined synthetic medium in the presence of (14)C-glucose to produce radio-labelled cells. Adherence to lipids was tested using a thin layer chromatography plate-based assay. SIGNIFICANCE AND IMPACT OF THE STUDY: The mechanism of bacterial adhesion to skin lipids is unknown. The results of this study show that a significant proportion of cutaneous aerobic coryneform isolates from the axilla interact with skin lipids resulting in increased adherence, which may contribute to skin colonization. 相似文献
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We investigated the interaction of mercuric compounds with the bacteriaCorynebacterium ammoniagenes, Micmcoccus luteus, andMycobacterium smegmatus capable of producing hydroxy lamines (R-NOH) and 2-C-methyl-D-erythritol-2,4-cyclopvrophosphate (MECP), which are prone to
form free radicals. The interaction of these substances with Hg2+ ions and their dynamics during the mercuric poisoning of bacteria was studied by EPR and NMR. Under stress conditions induced
by lowering pH or generation of active oxygen species, the bacteria and, especially, their mutants with enhanced sensitivity
to oxidative stress, were found to respond to exposure to 1–3 μ/ml HgCl2 andp-chloromercuribenzoate by a several-fold increase in their viability. The data obtained were interpreted in terms of the involvement
of the sulfhydryl groups of bacterial surface proteins in this phenomenon. The interaction of bacteria with mercuric compounds
may affect the pathogenesis of tuberculosis and other diseases. 相似文献
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