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1.
S. Raj V. Vinod J. Jayakumar P. Suresh A. Kumar R. Biswas 《Letters in applied microbiology》2021,73(1):31-38
Candida species are opportunistic human fungal pathogens that cause acute and chronic infections against which only few antifungal agents are available. Here we have elucidated the antifungal effect of Syzygium samarangense leaf extracts (SSLE). Antifungal activity of SSLE was studied against Candida albicans, C. krusei, C. parapsilosis, C. glabrata, C. auris and C. tropicalis. Following experiments were performed: minimum fungicidal concentration (MFC) determination, agar well disc diffusion assays, fungal morphology analysis using scanning electron microscope (SEM), ex vivo fungal survival assays on porcine tongue and skin and in vivo fungal survival assays using Drosophila melanogaster fly model. Results demonstrated MFC of SSLE ranges between 100 and 125 mg ml−1. SEM images showed cell wall degradation of C. albicans when treated with SSLE. Around 75% decrease in C. albicans viability was observed when infected porcine tongue and skin were treated using SSLE. The C. albicans infected D. melanogaster when fed with SSLE showed significant decrease (around 80%) of fungal count than the infected control. Furthermore, agar plate disc diffusion assays demonstrated that the antifungal activity of SSLE could be due to chalcone, which is one of the active constituents in SSLE. Our study demonstrated that SSLE could be used for the topical treatment of Candida infections. 相似文献
2.
K.L. Chew S. Octavia R. Jureen R.T.P. Lin J.W.P. Teo 《Letters in applied microbiology》2021,73(3):286-293
The objective of the study was to evaluate the use of targeted multiplex Nanopore MinION amplicon re-sequencing of key Candida spp. from blood culture bottles to identify azole and echinocandin resistance associated SNPs. Targeted PCR amplification of azole (ERG11 and ERG3) and echinocandin (FKS) resistance-associated loci was performed on positive blood culture media. Sequencing was performed using MinION nanopore device with R9.4.1 Flow Cells. Twenty-eight spiked blood cultures (ATCC strains and clinical isolates) and 12 prospectively collected positive blood cultures with candidaemia were included. Isolate species included Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis and Candida auris. SNPs that were identified on ERG and FKS genes using Snippy tool and CLC Genomic Workbench were correlated with phenotypic testing by broth microdilution (YeastOne™ Sensititre). Illumina whole-genome-sequencing and Sanger-sequencing were also performed as confirmatory testing of the mutations identified from nanopore sequencing data. There was a perfect agreement of the resistance-associated mutations detected by MinION-nanopore-sequencing compared to phenotypic testing for acquired resistance (16 with azole resistance; 3 with echinocandin resistance), and perfect concordance of the nanopore sequence mutations to Illumina and Sanger data. Mutations with no known association with phenotypic drug resistance and novel mutations were also detected. 相似文献
3.
M. Gopalakrishnan P. Sureshkumar J. Thanusu V. Kanagarajan 《Journal of enzyme inhibition and medicinal chemistry》2013,28(3):347-351
Compound 26 is more potent against Escherichia coli. and 24 is more active against Staphylococcus aureus, β-Heamolytic streptococcus, Vibreo cholerae, Salmonella typhii, and Shigella flexneri than the standard drug ciprofloxacin. Moreover, of all the compounds tested, 26 is more effective against Aspergillus flavus and Mucor, than the standard drug fluconazole. 相似文献
4.
5.
Hans D. VanEtten 《Phytochemistry》1973,12(7):1791-1792
A second antifungal isoflavan has been isolated from diseased bean hypocotyls and identified as 2′-methoxyphascollinisoflavan. 相似文献
6.
Michael A. Borowitzka 《Journal of applied phycology》1995,7(1):3-15
In the last decade the screening of microalgae, especially the cyanobacteria (blue-green algae), for antibiotics and pharmacologically
active compounds has received ever increasing interest. A large number of antibiotic compounds, many with novel structures,
have been isolated and characterised. Similarly many cyanobacteria have been shown to produce antiviral and antineoplastic
compounds. A range of pharmacological activities have also been observed with extracts of microalgae, however the active principles
are as yet unknown in most cases. Several of the bioactive compounds may find application in human or veterinary medicine
or in agriculture. Others should find application as research tools or as structural models for the development of new drugs.
The microalgae are particularly attractive as natural sources of bioactive molecules since these algae have the potential
to produce these compounds in culture which enables the production of structurally complex molecules which are difficult or
impossible to produce by chemical synthesis. 相似文献
7.
8.
Deng-Sen Xiong Ya-Bin Yang Bin-Yuan Hu Cui-Ping Miao Yong-Liang Wang Jin-Mei Zou Lei Li Yi-Qing Li Xiao-Dong Luo Li-Xing Zhao 《化学与生物多样性》2021,18(3):e2000964
Endophytic fungi play important roles for host's stress tolerance including invasion by pathogenic microbes. Small molecules are common weapons in the microbe–microbe interactions. Panax notoginseng is a widely used traditional Chinese medicinal plant and harbors many endophytes, some exert functions against pathogens. Here, we report six new compounds named myrothins A–F ( 1 – 6 ) produced by Myrothecium sp. BS-31, an endophyte isolated from P. notoginseng, and their antifungal activities against pathogenic fungi causing host root-rot disease. Their structures were elucidated with analysis of spectroscopic data including 1D and 2D NMR, HR-ESI-MS. Myrothins B ( 2 ) and E ( 5 ) showed the weak activity against Fusarium oxysporum and Phoma herbarum, and myrothins F ( 6 ) showed weak activity against F. oxysporum. 相似文献
9.
10.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(6):1307-1315
An efficient and simple microwave assisted synthesis of sulfonamide derivatives incorporating the pyridazine moiety has been developed. These sulfonamides were used for the preparation of new heterocyclic compounds via reaction with different reagents using a microwave irradiation technique. The structures of the newly synthesized compounds were confirmed on the basis of FTIR, 1H and 13C-NMR, mass spectral techniques and elemental analyses. Some of the new synthesized compounds were assayed for their in vitro antibacterial activity against Gram-positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, Gram-negative bacteria, Escherichia coli and Klebsiella pneumonia and antifungal activity against Aspergillus fumigatus and Candida albicans. Most of the new compounds showed significant antibacterial and antifungal activity. 相似文献