Response of miRNAs and their targets to salt and drought stresses in cotton (Gossypium hirsutum L.)

MicroRNAs (miRNAs) are an important gene regulator, controlling almost all biological and metabolic processes, in both plants and animals. In this study, we investigated the effect of drought and salinity stress on the expression of miRNAs and their targets in cotton (Gossypium hirsutum L.). Our results show that the expression change of miRNAs and their targets were dose-dependent and tissue-dependent under salinity and drought conditions. The expression of miRNAs in leaf was down-regulated under higher salinity stress while shows variable patterns in other conditions. The highest fold-changes of miRNAs were miR398 in roots with 28.9 fold down-regulation under 0.25% NaCl treatment and miR395 in leaves with 7.6 fold down-regulation under 1% PEG treatment. The highest up-regulation of miRNA targets was AST in roots with 4.7 fold-change under 2.5% PEG and the gene with highest down-regulation was CUC1 in leaves with 25.6 fold-change under 0.25% NaCl treatment. Among seven miRNA-target pairs we studied, five pairs, miR156–SPL2, miR162–DCL1, miR159–TCP3, miR395–APS1 and miR396–GRF1, show significant regulation relationship in roots and leaves under salinity stress concentration.     

中国鳞刺蛾属订正(鳞翅目,刺蛾科)(英文)

经过系统研究,发现我国的鳞刺蛾并不是一直被称为的眼鳞刺蛾Squamosa ocellata(Moore),共有2种和1亚种.首次报道了姹鳞刺蛾S.chalcites Orhant在我国有分布,描述了1新种,短爪鳞刺蛾S.brevisunca和1新亚种,云南亚种S.brevisunca yunnanensis.本文还描述了云南亚种的茧和老熟幼虫形态,编制了分种和亚种检索表,提供了成虫彩色照片和外生殖器特征图.模式标本保存在中国科学院动物研究所国家动物博物馆.