A molecular biogeographic analysis of the relationship between North American melanoploid grasshoppers and their Eurasian and South American relatives

The Melanoplinae constitute one of the two largest subfamilies of Acrididae. Distributed mainly throughout the New World and parts of Eurasia, this group of grasshoppers includes over 100 genera and 800 species. Over the past five decades there has been considerable speculation on the origins of North and South American taxa. The most favored hypothesis proposes an ancient division of Laurasian taxa accompanying the separation of North America and Eurasia, with subsequent radiations within those continents, followed by a recent incursion of Nearctic melanoplines into the southern hemisphere with the establishment of the Isthmus of Panama. This research tests that scenario by phylogenetic analysis using as characters portions of five mitochondrial gene sequences, totaling 2285 bp. Three tree-building methods, maximum-parsimony, neighbor-joining, and maximum-likelihood, strongly support the different view that melanopline grasshoppers originated somewhere in the Americas and spread to the Old World. The feasibility of these findings is discussed within a geological context.     

A DNA investigation into the mysterious disappearance of the Rocky Mountain grasshopper, mega-pest of the 1800s

The mysterious extinction of the Rocky Mountain Grasshopper, Melanoplus spretus, a major pest species on the prairies and plains of the 1800s, is truly a remarkable event in the history of agriculture. Recently, we obtained specimens of M. spretus from museums and from 400-year-old glacial deposits in Wyoming. We report success in obtaining mitochondrial DNA sequences from both sources. This permitted us to examine two issues surrounding the species' disappearance. First, a long-standing view that M. spretus and the extant species Melanoplus sanguinipes are (if not phase transforms of one another) sister taxa, is disputed, but cannot be rejected with certainty. Interestingly, there is some evidence that suggests there may be a closer affinity with another member of the Mexicanus species-group, Melanoplus bruneri. Second, because M. spretus still possesses considerable nucleotide diversity (1.15+/-0.19%), a depletion of variation cannot be considered a factor contributing to its demise.     

Tests of pleistocene speciation in montane grasshoppers (genus Melanoplus) from the sky islands of western North America

There has a been a resurgence of debate on whether the Pleistocene glaciations inhibited speciation. This study tests a model of Pleistocene speciation, estimating the phylogenetic relationships and divergence times of 10 species of montane grasshoppers, genus Melanoplus, using 1300 bp of the mitochondrial gene cytochrome oxidase I (COI). Based on average pairwise distances (corrected for multiple substitutions using Kimura's two-parameter model), all species appear to have originated within the Pleistocene. Sequence divergences between species are less than 4%, corresponding to divergence times less than 1.7 million years ago. Branching patterns among the species suggest that speciation was associated with more than one glacial-interglacial cycle. A likelihood-ratio test rejected a model of simultaneous species origins, the predicted branching pattern if species arose from the fragmentation of a widespread ancestor. These grasshoppers live in an area that was previously glaciated and, as inhabitants of the northern Rocky Mountain sky islands, underwent latitudinal and probably altitudinal shifts in distribution in response to climatic fluctuations. Given the repeated distributional shifts and range overlap of the taxa, there most likely has been ample opportunity for population mixing. However, despite periodic glacial cycles, with more than 10 major glaciations over the past million years and climatic fluctuations over as short a time scale as 10(3) to 10(4) years, the dynamic history of the Pleistocene did not preclude speciation. Although relationships among some taxa remain unresolved, these grasshopper species, even with their recent origins, exhibit genetic coherence and monophyletic or paraphyletic gene trees. The frequency of glacial cycles suggests that the speciation process must have been extremely rapid. These species of grasshoppers are morphologically very similar, differing primarily in the shape of the male genitalia. These characters are posited to be under sexual selection, may play an important role in reproductive isolation, and are known to diverge rapidly. This suggests the rapidity of evolution of reproductive isolation may determine whether species divergences occurred during the Pleistocene glaciations.     

Two Species of Melanoplinae (Orthoptera: Catantopidae) New to Korea

ABSTRACT Two species of the subfamily Melanoplinae (Orthoptera: Catantopidae) from Korean peninsular are newly recognized: Podisma aberrans Ikonnikov and Miramella solitaria (Ikonnikov).