Observations on the meat-eating behavior of wild bonobos (Pan paniscus) at Wamba,Republic of Zaire

Meat-eating behavior of wild bonobos (Pan paniscus) was witnessed on two occasions at Wamba, Republic of Zaire. Only flying squirrels were observed to be eaten by the bonobos. Several bonobos gathered around the possessor of the meat and showed interest in the meat on all occasions. Begging behavior was noted on one of the two occasions, but the possessor of the meat ignored it. No sharing of meat was seen on either occasion. The exclusive targets of hunting by bonobos are apparently small mammals, such as flying squirrels and infant duikers, since evidence of meat eating by wild bonobos, which have been studied for more than fifteen years, has been restricted to these mammals. The bonobos at Wamba may have a specialized “prey image”, as in the case of the chimpanzees (Pan troglodytes) of the Tai forest, and certain medium-sized or small mammals may not conform to this image.     

Genome-wide association study reveals a quantitative trait locus and two candidate genes on Sus scrofa chromosome 5 affecting intramuscular fat content in Suhuai pigs

Intramuscular fat content (IFC) is an essential quantitative trait of meat, affecting multiple meat quality indicators. A certain amount of IFC could not only improve the sensory score of pork but also increase the flavour, tenderness, juiciness and shelf-life. To dissect the genetic determinants of IFC, two methods, including genome-wide efficient mixed-model analysis (GEMMA) and linkage disequilibrium adjusted kinships (LDAKs), were used to carry out genome-wide association studies for IFC in Suhuai pig population. A total of 14 and 18 significant single nucleotide polymorphisms (SNPs) were identified by GEMMA and LDAK, respectively. The results of these two methods were highly consistent and all 14 significant SNPs in GEMMA were detected by LDAK. Seven of the 18 SNPs reached the genome-wide significance level (P < 9.85E−07) while 11 cases reached the suggestive significance level (P < 1.77E−05). These significant SNPs were mainly distributed on Sus scrofa chromosome (SSC) 5, 3, and 7. Moreover, one locus resides in a 2.27 Mb (71.37–73.64 Mb) region on SSC5 harbouring 13 significant SNPs associated with IFC, and the lead SNP (rs81302978) also locates in this region. Linkage disequilibrium (LD) analysis showed that there were four pairs of complete LD (r² = 1) among these 13 SNPs, and the remaining 9 SNPs with incomplete LD (r² ≠ 1) were selected for subsequent analyses of IFC. Association analyses showed that 7 out of 9 SNPs were significantly associated with IFC (P < 0.05) in 330 Suhuai pigs, and the other 2 SNPs tended to reach a significant association level with IFC (P < 0.1). The phenotypic variance explained (PVE) range of these 9 SNPs was 0.92–3.55%. Meanwhile, the lead SNP was also significantly associated (rs81302978) with IFC (P < 0.05) in 378 commercial hybrid pigs (Pietrain × Duroc) × (Landrace × Yorkshire) (PDLY), and the PVE was 1.38%. Besides, two lipid metabolism-relevant candidate genes, the leucine rich repeat kinase 2 (LRRK2) and PDZ domain containing ring finger 4 (PDZRN4) were identified in the 2.27 Mb region on SSC5. In conclusion, our results may provide a set of markers useful for genetic improvement of IFC in pigs and will advance the genome selection process of IFC on pig breeding programmes.     

Multi-test analysis and model-based estimation of the prevalence of Taenia saginata cysticercus infection in naturally infected dairy cows in the absence of a ‘gold standard’ reference test

The diagnostic values of seven serological tests (ELISAs) and of the obligatory European Union-approved routine visual meat inspection for the detection of Taenia saginata cysticercosis were investigated. A total of 793 slaughtered dairy cows were selected in three European Union approved abattoirs in Switzerland, an endemic area (apparent prevalence by enhanced meat inspection up to 4.5%) with typically low parasite burdens. ELISAs based on a somatic larval antigen, isoelectric focused somatic larval antigen, larval excretory/secretory antigens, peptide HP6-2, peptide Ts45S-10, pooled peptide solution and a monoclonal antibody antigen capture assay were initially screened. As there is no perfect diagnostic ‘gold standard’ reference test, the obligatory meat inspection and four selected serological tests were further analysed using Bayesian inference to estimate the “true” prevalence and the diagnostic test sensitivities and specificities. The ELISA for specific antibody detection based on excretory/secretory antigens showed highest sensitivity and specificity with 81.6% (95% credible interval: 70–92) and 96.3% (95% credible interval: 94–99), respectively. The Bayesian model estimated the specificity of the ELISA, based on the synthetic peptide Ts45S-10 as 55.2% (95% credible interval: 46–65) and sensitivity as 84.7% (95% credible interval: 82–88). The sensitivity of the ELISA based on mAbs, detecting circulating antigen, was 14.3% (95% credible interval: 9–23) with a specificity of 93.7% (95% credible interval: 92–96). The diagnostic sensitivity of the obligatory standard European Union meat inspection procedure for the detection of T. saginata cysticercus infection at the abattoir was estimated to be 15.6% (95% credible interval: 10–23). Based on these data, the modelled prevalence of cysticercosis in dairy cows presented at abattoirs in Switzerland was estimated to be 16.5% (95% credible interval: 13–21). These cattle also had a high prevalence of infection with Dicrocoelium dendriticum (60.8%) and Fasciola hepatica (13.5%).     

从微生物指标中看我国肉类食品安全问题及解决措施

本研究从食品微生物指标人手,对我国肉类食品的微生物指标检测情况及其产品质量进行分析,在相关政府部门的有力的监管下,肉类产品质量与安全有了较大幅度提高.最后分析了我国肉类食品行业的现存问题,并提出改进的建议,最后对其发展进行展望.     

Untersuchungen über die Qualität des Futtereiweißes bei Legehennen

An experiment with 40 female growing pigs from 27.4 to 106.5 kg body weight (BW) in individual pens was conducted to evaluate the effect of different chromium supplements (Cr‐chloride, Cr‐yeast and Cr‐picoli‐nate) according to 0.5 ppm Cr in the diet compared with a control diet without any additional chromium. The influence on growth performance and carcass as well as meat composition was studied.

In comparison with the control diet body weight gain and feed conversion ratio in the finishing period (60 to 106.5 kg BW) were significant increased and lowered respectively in the treatment with Cr‐chloride and with the other Cr supplements tendentially. The results of the carcass composition as well as the fatty acid profile of neutral and complex lipids in the muscle (M. longissimus dorsi) at the 10th rib did not indicate a statistically significant effect of the Cr supplements. Furthermore energy utilization on the base of digestibility was not affected. Concerning the Longissimus muscle area and the intramuscular fat content there were positive tendencies of the investigated Cr supplements.     

Growth performance,carcass characteristics and bioavailability of isoflavones in pigs fed soy bean based diets

A growth trial with 38 weaners (castrated male pigs) was designed to compare the growth performance and carcass quality of pigs fed diets containing either soy bean meal or soy protein concentrate in a pair-feeding design. Soy bean meal (SBM) and soy protein concentrate (SPC) differed in isoflavone (daidzein plus genistein) content (782?μg/g in SBM and 125?μg/g in SPC, respectively). During the experiment, all pigs were fed four-phases-diets characterized by decreasing protein concentrations with increasing age (weaner I, weaner II, grower, finisher diets). Rations of control and experimental groups were isoenergetic, isonitrogenous, and isoaminogen. The weanling pigs with an initial live weight of 8.4?±?1.1?kg were allotted to flat deck boxes. During the growing/finishing period (days 70?–?170 of age), the pigs were housed in single boxes. Both, the weaning and the grower/finishing performances (daily body weight gain, feed intake, feed conversion ratio) were similar in both groups. No differences were found between the groups in carcass composition (percentages of cuts, tissues, and protein/fat), and meat quality of pigs. Moreover, the IGF-1R mRNA expression in longissimus muscle was not influenced by the kind of soy product. However, circulating levels of isoflavones were clearly different between pigs fed SBM (genistein 239?±?44; daidzein 162?±?42; equol 12?±?4?ng/ml plasma) and animals fed SPC (genistein 22?±?9 and daidzein 8?±?3, and equol 10?±?3?ng/ml plasma). The results confirm the expected differences in the bioavailability of soy isoflavones, yet, there were no significant differences in performance of pigs fed either soy bean meal or soy protein concentrate.     

兴义鸭LPL基因外显子8多态性与生长和肉质性状的关联性分析

脂蛋白脂酶(LPL)是动物脂质沉积和新陈代谢的关键酶,对生长和肉质发挥着重要作用。试验采用PCR-SSCP法和DNA直接测序技术相结合对兴义鸭L PL基因外显子8进行多态性检测,并分析其多态与生长和肉质性状的关联性。结果表明,在兴义鸭L PL基因外显子8首次检测到1个T1251C同义突变,产生三种基因型TT、TC和CC,2个等位基因T和C,基因型TT和等位基因T的频率分别为0.8077和0.8750,多态信息含量为0.1948,表现为低度多态,卡方(字2)检验表明T1251C位点的基因型分布在兴义鸭中未偏离Hardy-Weinberg平衡。最小二乘分析显示,TT基因型个体的宰前体重显著低于CC基因型(p<0.05),胸宽显著低于TC基因型(p<0.05),推测等位基因C可能是兴义鸭生长性状的有利等位基因,可作为生长性状的一个标记性辅助选择位点。     

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

We describe a simple protocol for identifying and quantifying the two components in binary mixtures of species possessing one or more similar proteins. Central to the method is the identification of ''corresponding proteins'' in the species of interest, in other words proteins that are nominally the same but possess species-specific sequence differences. When subject to proteolysis, corresponding proteins will give rise to some peptides which are likewise similar but with species-specific variants. These are ''corresponding peptides''. Species-specific peptides can be used as markers for species determination, while pairs of corresponding peptides permit relative quantitation of two species in a mixture. The peptides are detected using multiple reaction monitoring (MRM) mass spectrometry, a highly specific technique that enables peptide-based species determination even in complex systems. In addition, the ratio of MRM peak areas deriving from corresponding peptides supports relative quantitation. Since corresponding proteins and peptides will, in the main, behave similarly in both processing and in experimental extraction and sample preparation, the relative quantitation should remain comparatively robust. In addition, this approach does not need the standards and calibrations required by absolute quantitation methods. The protocol is described in the context of red meats, which have convenient corresponding proteins in the form of their respective myoglobins. This application is relevant to food fraud detection: the method can detect 1% weight for weight of horse meat in beef. The corresponding protein, corresponding peptide (CPCP) relative quantitation using MRM peak area ratios gives good estimates of the weight for weight composition of a horse plus beef mixture.     

The chemical composition of the meat of fat dormice (<Emphasis Type="Italic">Glis glis</Emphasis> L.)

The fat dormouse (Glis glis) is a traditional game species in the Republic of Croatia. Although today the fat dormouse is not frequently caught as game, it is still a source of animal protein in human nutrition in certain rural areas of Croatia. In this paper the chemical analysis of fat dormouse meat is presented. The average values for the quantity of water, fat, protein and ash in dormouse meat are comparable with the chemical composition of the meat of rabbits and brown hares, except for the important fact that rabbit and hare meat contains a greater quantity of fat on average. According to our results, the meat of fat dormice can be categorised as dietary food, characterised by a small percentage of fat (mean 2.83%) and a high amount of protein (mean 21.01%).