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A new stain for identification of avian leukocytes 总被引:3,自引:0,他引:3
Differential staining of avian leukocytes was achieved within 6 min following brief fixation in a methanolic solution of C.I. acid red 360 followed by immersion in a mixture containing C.I. basic blue 41, C.I. basic blue 141, and C.I. acid red 52. Heterophils contained black angular and punctate granules. Eosinophils contained bright purple granules. Lymphocytes displayed red nuclei and blue cytoplasm. Monocytes contained red-brown nuclei and lavender cytoplasm. Basophils showed red-orange granules. Thrombocytes stained deep purple. Compared to traditional panoptic stains like Wright's or Giemsa's, the new staining method provides brighter colors, more precise details of cellular structures, and shorter staining time. Significantly, it facilitates identification of avian leukocyte species based on differences in color as well as differences in size and shape. 相似文献
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van der Heijden KM Szabó MP Egami MI Pereira MC Matushima ER 《Experimental & applied acarology》2005,37(3-4):245-255
In the present work features of tick-bite lesions were evaluated in capybaras naturally infested with Amblyomma cajennense and Amblyomma dubitatum ticks. Gross appearance of tick bite site was characterized by a mild swelling and erythema. Microscopic examination revealed
the cement cone, a tube-like homogenous eosinophilic mass penetrating deep into the dermis. This structure was surrounded
in the dermis by a cellular infiltrate and free eosinophilic granules and was associated to edema of variable intensity. Necrosis
was a common feature deep in the dermis particularly at the far end of the eosinophilic tube. Hyperplasia, cellular edema
and occasionally necrosis of keratinocytes could be seen at both sides of the ruptured epidermis. Cellular infiltrate was
constituted overwhelmingly by polymorphonuclear leukocytes with eosinophilic granules. In capybaras cells with such features
can be either eosinophils or heterophils (pseudoeosinophils), the latter being the equivalent of neutrophils of other mammals.
Ultrastructural analysis of the cellular infiltrate revealed the predominance of heterophils over eosinophils. Mononuclear
cells and mast cells and, in lesser numbers, basophils were also seen at skin attachment sites. The presence of heterophils
in the reaction of capybaras against Amblyomma ticks is an outstanding feature but its role in the reaction to the tick is not known. It is however speculated that capybara
heterophils might be associated with a more permissive environment for tick feeding and pathogen transmission as already shown
for the equivalent cell type, the neutrophil, in the reaction of the dog against the Rhipicephalus sanguineus tick. 相似文献
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《Biotechnic & histochemistry》2013,88(4):201-206
Differential staining of avian leukocytes was achieved within 6 min following brief fixation in a methanolic solution of C.I. acid red 360 followed by immersion in a mixture containing C.I. basic blue 41, C.I. basic blue 141, and C.I. acid red 52. Heterophils contained black angular and punctate granules. Eosinophils contained bright purple granules. Lymphocytes displayed red nuclei and blue cytoplasm. Monocytes contained red-brown nuclei and lavender cytoplasm. Basophils showed red-orange granules. Thrombocytes stained deep purple. Compared to traditional panoptic stains like Wright's or Giemsa's, the new staining method provides brighter colors, more precise details of cellular structures, and shorter staining time. Significantly, it facilitates identification of avian leukocyte species based on differences in color as well as differences in size and shape. 相似文献
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