扦插玉米秸秆改良松嫩平原次生光碱斑的研究

根据野外实验,提出了改良松嫩平原次生光碱斑的新方法,即通过扦插玉米秸秆截留植物种子,在玉米秸秆分解作用下,以玉米秸秆本身及其邻近区域,为植物提供生长平台,使被截留的植物种子得以顺利定居、生长;达到低成本、快速地恢复次生光碱斑植被的日的．结果表明,扦插玉米秸秆可显著提高土壤种子库,改良区土壤种子数量为4020．0±1773．6粒·m^2,次生光碱斑土壤种子库为10．0±31．6粒·m^2,被截留的种子为植被恢复提供了种源．改良区土壤理化特性得到一定改善,但仍具有高pH值、高盐分含量和低有机质含量等特征．虎尾草能在玉米秸秆周围存活,每个玉米秸秆周围可生长3．9±2．2株,产量可达68．64±38．72g·m^-2．该方法投入少、成本低、技术简单,在次生光碱斑呈斑块状、且面积相对较小的区域．具有更大的推广潜力．     

Simultaneous production of propionic acid and vitamin B12 from corn stalk hydrolysates by Propionibacterium freudenreichii in an expanded bed adsorption bioreactor

Abstract

Vitamin B12 and propionic acid that were simultaneous produced by Propionibacterium freudenreichii are both favorable chemicals widely used in food preservatives, medicine, and nutrition. While the carbon source and propionic acid accumulation reflected fermentation efficiency. In this study, using corn stalk as a carbon source and fed-batch fermentation process in an expanded bed adsorption bioreactor was studied for efficient and economic biosynthesis of acid vitamin B12 and propionic. With liquid hot water pretreated corn stalk hydrolysates as carbon source, 28.65?mg L^?1 of vitamin B12 and 17.05?g L^?1 of propionic acid were attained at 168?h in batch fermentation. In order to optimize the fermentation outcomes, fed-batch fermentation was performed with hydrolyzed corn stalk in expanded bed adsorption bioreactor (EBAB), giving 47.6?mg L^?1 vitamin B12 and 91.4?g L^?1 of propionic acid at 258?h, which correspond to product yields of 0.37?mg g^?1 and 0.75?g g^?1, respectively. The present study provided a promising strategy for economically sustainable production of vitamin B12 and propionic acid by P. freudenreichii fermentation using biomass cornstalk as carbon source and expanded bed adsorption bioreactor.