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S. J. Ochatt E. M. Patat-Ochatt E. L. Rech M. R. Davey J. B. Power 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1989,78(1):35-41
Summary Mesophyll protoplasts of wild pear (Pyrus communis var. pyraster L., Pomoideae) were chemically fused with cell suspension protoplasts of cherry rootstock Colt (Prunus avium x pseudocerasus, Prunoideae), following an electroporation treatment of the separate parental protoplast systems. Fusion-treated protoplasts were cultured, on modified K8P medium, where it had been previously established that neither parental protoplasts were capable of division. Somatic hybrid calli were recovered and, following caulogenesis on MS medium with zeatin and after rooting of regenerated shoots, complete trees were obtained and grown in vivo. Hybridity of these trees was confirmed based on morphological characters, chromosome complement and isozyme analysis. Two separate cloned lines of this intersubfamilial rootstock somatic hybrid (wild pear (+) Colt cherry) were produced. This is the first report of the production of somatic hybrid plants of two woody species, of agronomic value, within the order Rosales. 相似文献
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Hairy roots were obtained after inoculation with Agrobacterium rhizogenes strain NCPPB 1855 of the in-vitro-grown shoots of the cherry rootstocks Colt (Prunus avium×P. pseudocerasus) and Mazzard F12/1 (P. avium L.). Not all putatively transgenic roots were able to grow in hormone-free medium. Mazzard F12/1 roots, induced with A. rhizogenes, did not differentiate any shoot or embryo, while both somatic embryos and shoots differentiated from the transgenic roots
of Colt in medium containing 1 mg/l 6-benzylaminopurine and 1 mg/l 1-naphthaleneacetic acid. Somatic embryos were capable
of secondary embryogenesis, but few developed into whole plants. DNA hybridization showed both a different number of bands
and signal intensity in each of the five transgenic shoot clones and embryos examined. In a morphogenetic in vitro test, leaf
explants of the transgenic shoot clones showed an increased capacity to differentiate roots, although clones differed in their
sensitivity to the hormone ratio. Clones from the transgenic shoots had not only an increased rooting ability when grown in
vitro but also exhibited various hairy root phenotypes when cultured in vitro and when transferred into the greenhouse.
Received: 12 December 1996 / Revision received: 21 March 1997 / Accepted: 10 May 1997 相似文献
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