Neurite-promoting activities for embryonic spinal neurons and their developmental changes in the chick

Spinal motoneurons may depend upon muscle-derived factors for axon outgrowth and stabilization at two principal stages of their development: during the initial invasion of the differentiating muscle masses in the embryo and during the perinatal regression of multiple innervation. Using a bioassay involving the measurement of neurite outgrowth from 4.5-day embryonic chick spinal neurons in dissociated cell culture, neurite-promoting activities were detected both in medium conditioned over embryonic chicken myotubes in vitro (embryonic muscle-conditioned medium) and in soluble extracts of chick leg muscle prepared 3-5 days after hatching (postnatal muscle extract). The molecules responsible for these two activities had physicochemical properties that distinguished them both from each other and from some other reported neurite-promoting factors. The factor in embryonic muscle-conditioned medium, although active on uncoated tissue culture wells, bound with only low affinity to tissue culture plastic under cell culture conditions. It was inactivated by incubation with trypsin, and was essentially found only in media conditioned by muscle and liver cells. The factor in PNME, on the other hand, bound to plastic culture wells and was found in extracts of a variety of tissues. Its concentration in postnatal leg muscle was developmentally regulated: the specific activity increased approximately 10-fold between hatching and Day 3 (maximum value: 3200 units/mg protein) and then fell back to nearly its original levels by Day 7. Evidence is presented that the observed effects of these two neurite-promoting factors did not result from differential survival in vitro of different cell subpopulations. Possible roles for the two active factors during motoneuron development are discussed.     

东方扁虾雄性生殖系统的解剖学和组织学研究

东方扁虾雄性生殖系统由精巢、输精管及雄性生殖孔三部分组成,输精管可分为前、中、后三段。精巢由卷绕的前、后收集管及持靠其上的许多生精腺囊所组成。同一腺囊内的精细胞发生基本同步,而不同腺囊内则可以不同步。收集管的主要功能是将精细胞团输送至输精管。精荚在输粗管内运行时一直进行着精子的形成过程,直至精子成熟。位于输精管末段不肌层外的索带状细胞团被认为是造雄腺。     

Extrasynaptic localization of α-bungarotoxin receptors in the rat superior cervical ganglion

Binding of [¹²⁵I]α-bungarotoxin to nicotinic cholinergic receptors (α-bungarotoxin receptors) was investigated in the rat superior cervical ganglion by light and electron microscope autoradiography. Both techniques indicated that labelling, which was inhibited by d-tubocurarine, occurred around and/or over neuronal perikarya. In particular, ultrastructural autoradiography showed that the synapses were devoid of radioactivity, suggesting that α-bungarotoxin receptors in the rat superior cervical ganglion are molecules distinct from the nicotinic (postsynaptic) receptors normally involved in ganglionic transmission. By contrast, specific labelling was found in extrasynaptic areas of the neuronal membrane in contact with satellite cells (neuron-satellite cell boundary). Quantitative analysis indicated that at that level silver grains were present on both the neuronal membrane and satellite cells. Furthermore, beside neuronal perikarya, radioactivity was also found around nerve fibres, probably in relation to both the axonal and interstitial sides of the ensheathing Schwann cells. Only a few grains were clearly accumulated inside nerve fibres. Finally, significant amounts of specific radioactivity were detected in the neuronal cytoplasm, especially at the level of rough endoplasmic reticulum and Golgi apparatus. However, parallel diffusion experiments with [¹²⁵I]α-bungarotoxin and [³H]inulin (a marker for the extracellular space) provided no evidence that the toxin enters the neuronal cytoplasm. Thus, the intraneuronal (specific) labeling was probably a reflection of α-bungarotoxin binding to membrane receptors and the subsequent internalization of the toxin-receptor complex in the neurons. We conclude that in the rat superior cervical ganglion extrasynaptic nicotinic acetylcholine receptors (α-bungarotoxin receptors) may be widely located on the neuronal membrane as well as on the plasma membrane of satellite and Schwann cells. The physiological significance of this molecular architecture is discussed.