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影响白腐真菌AH28-2菌株漆酶合成的因子和发酵条件   总被引:12,自引:0,他引:12       下载免费PDF全文
White-rot fungus AH28-2, a newly isolated strain, produced effectively laccase by induction when grown on a synthetic medium. Aromatic compounds of low molecular weight had an inducing influence on laccase production and its isoenzyme compositions. The using of o-toluidine or syringic acid had the best inducing effect. Cu2+ concentration in medium had distinguished effect on laccase production. Enzyme activity was notably increased by Cu2+ and reached the maximum when Cu2+ final concentration was 5 mumol/L. Mn2+ inhibited the synthesis of laccase. Carbon and nitrogen limitation were not beneficial to laccase synthesis, while high nutrient organic medium was beneficial to the growth of cell and the synthesis of laccase. Using cellobiose as the sole carbon source, the highest level enzyme activity reached 82,923. 7 u/L under the condition of optimum fermentation with ABTS as substrate. This enzyme activity was 2.9-fold higher compared to the reported data on international references in recent years.  相似文献
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白腐真菌AH28-2菌株发酵合成漆酶初步研究   总被引:12,自引:0,他引:12  
从224个野外采集的真菌样品中筛选分离到一株产漆酶活性较高菌株AH28-2,经初步鉴定为白腐真菌,采用单因子相互比较法,研究了该菌株最适发酵产酶条件。应用添加有1g/LKraft木素的液体发酵培养基,接种量5%(V/V),初始pH8.5,装液量为50%,28℃、150r/min摇瓶振荡培养4-5d,漆酶酶活水平达20184IU/L。  相似文献
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