纽荷尔脐橙CCS基因的克隆与分析

利用RT-PCR技术从纽荷尔脐橙果实cDNA中分离出辣椒红／辣椒玉红素合成酶（Capsanthin/Capsorubin synthase,CCS）基因（Ccs）全长。该片断长为1619bp,编码503个氨基酸。BLAST比较其氨基酸同源性发现,该序列所推导的氨基酸与已知的甜橙CCS完全一致,与胡萝卜和辣椒的CCS,以及马铃薯新黄质合成酶（neoxanthin synthase,NSY）有70％的同源性。此外,与番茄和柑橘等的番茄红素环化酶也有50%~70%的同源性。半定量RT-PCR分析表明,Ccs在纽荷尔脐橙果实成熟过程中呈先上升后下降的表达趋势,期间9月份的表达量最高。     

甜橙辣椒红／辣椒玉红素合成酶同源基因的克隆（简报）

The complete sequence of orange homologous capsanthin/capsorubin synthase gene is 3788 bp long with a coding sequence of 1512 bp, which encodes a polypeptide of 503 amino acids. The 5' upstream sequence is 1721 bp long and the 3' downstream sequence is 555 bp long. The amino acid sequence of this gene is 78% and 69% identical to the genes from carrot and pepper, respectively. It is also partially homologous to plant neoxanthin synthase, lycopene beta-cyclase and lycopene epsilon cyclase genes. Isolation of the gene provides a framework for elucidation of the mechanisms involved in inability of citrus to produce capsanthin and capsorubin.     

甜橙辣椒红/辣椒玉红素合成酶同源基因的克隆(简报)

类胡萝卜素是由8个类异戊二烯单位组成的一类碳氢化合物及其氧化衍生物。它存在于所有植物中,并在光合作用及光保护等生理过程中起着重要作用。同时,类胡萝卜素也使果实呈现各种色泽。柑桔成熟果实的果皮及果汁色泽也主要是由于类胡萝卜素引起。果皮中类胡萝卜素种类及含量决定了