Characterization and mapping of a new male sterility mutant of anther advanced dehiscence （t） in rice

Anther dehiscence is very important for pollen maturation and release.The mutants of anther dehiscence in rice (Oryza sativa L.) arefew,and related research remains poor.A male sterility mutant of anther dehiscence in advance,add(t),has been found in Minghui 63 and its sterility is not sensitive to thermo-photo.To learn the character of sterilization and the function of the add(t) gene,the morphological and cytological studies on the anther and pollen,the ability of the pistil being fertilized,inheritance of the mutant,and mapping of add(t)gene have been conducted.The anther size is normal but the color is white in the mutant against the natural yellow in the wild-type.The pollen is malformed,unstained,and small in the KI-I2 solution.The anther dehiscence is in advance at the bicellular pollen stage.A crossing test indicated that the grain setting ratio of the add(t) is significantly lower than that of the CMS line 2085A.The ability of the pistil being fertilized is most probably decreased by the add(t) gene.The male sterility is controlled by a single recessive gene of add(t).This gene is mapped between the markers of R02004 (InDel) and RM300 (SSR) on chromosome 2,and the genetic distance from the add(t) gene to these markers is 0.78 cM and 4.66 cM,respectively.     

改良双侧V-Y皮瓣治疗Ⅳ期骶尾部压疮的疗效分析

摘要 目的：探讨改良双V-Y皮瓣治疗Ⅳ期骶尾部压疮的有效性和安全性。方法：回顾性分析2015年3月-2019年3月中国人民解放军总医院第七医学中心骨科收治骶尾部压疮Ⅳ期患者28例,其中男15例,女13例,年龄49-65岁,创面面积6 cm×7 cm~8 cm×10 cm。彻底清创后设计改良双侧V-Y推移皮瓣覆盖创面并行负压封闭引流,皮瓣供区减张缝合,皮瓣面积6 cm×7 cm~8 cm×10 cm。术后观察皮瓣整体血运、温度、水肿情况及VSD引流量,定期通过门诊、微信、电话随访。结果：手术后双侧V-Y皮瓣全部成活,1例术后切口周边皮肤表皮坏死,经换药后愈合,28例患者全部获得随访,随访时间12-24个月,平均18.2个月,全部压疮缺损修复成功,无创口裂开、皮瓣淤血、水肿、感染及压疮复发。结论：改良双侧V-Y推移皮瓣是治疗Ⅳ期骶尾部压疮的一种有效治疗方法。     

老年人腹部手术术后切口裂开的原因及对策

目的：探讨老年人腹部手术术后切口裂开的原因,提出防范措施。方法：回顾性分析956例60岁以上腹部手术病人的临床资料。结果：共有26例切口裂开,再次缝合后无一例再裂开。3例死亡,病死率为11．5％。结论：注意缝合技术和增加组织抗张力的强度,降低腹压,避免危险因素和对危险因素的围手术期处理,能有效地预防了老年人腹部手术术后的切口裂开。     

A Comparison Studies on Ultrastructure and Calcium Distribution in Fertile and Sterile Anthers of a Genic Male-sterile Rapeseed （Brassica napus L.）

It‘s common understanding that plant male sterility is closely related to cell ultrastructure or cell microstructure,plant physiological and biochemical metabolism during the generation and development of anthers. The materials used for the study were fertile and sterile anthers in various stages of a genic male-sterile rapeseed     

药用植物阳春砂花器形态特征

通过解剖阳春砂(Amomum villosum)小花,以体视镜观察并测量长果、圆果和仲华3个阳春砂栽培类型小花的形态指标。结果表明,仲华的假合蕊柱与唇瓣间的纵向距离最小,但3个栽培类型之间的横向距离差异不显著。假合蕊柱与唇瓣的距离大小主要由假合蕊柱的挺直程度决定,角度γ值是栽培类型的特征参数。仲华的花粉囊最长,长果次之,圆果最小;而3个栽培类型间的花粉囊宽度差异不显著;花粉囊长度的差异是栽培类型的另一个特征。仲华柱头的开口面积与长果和圆果差异不显著;但长果柱头的开口面积比圆果大。3个栽培类型柱头的开口均高于花粉囊顶端;圆果柱头的开口与花粉囊顶端的距离(Dsp)显著大于长果和仲华,而长果与仲华的Dsp差异不显著。该研究建立了阳春砂花器形态的研究方法,比较了3个栽培类型小花的形态特征,为寻找适宜的传粉途径和提高产量奠定了基础。