辽宁省菜田生态系统组分优势值与系统优势度分析

辽宁省菜田生态系统组分优势值与系统优势度分析周宝利，葛晓光李宁义（沈阳农业大学园艺系，１１０１６１）（沈阳市城建中专，１１００１３）ＡｎａｌｙｓｉｓｏｎＳｕｐｅｒｉｏｒｉｔｙＶａｌｕｅａｎｄＤｏｍｉｎａｎｃｅＤｅｇｒｅｅｏｆＶａｒｉｏｕｓＣｏｍｐｏｎ...     

建立以TK基因为标记将外源基因导入臭曲霉（Asper—gillus...

A new system for selection of transformed Aspergillus foetidus was reported. In this system, TK- A. foetidus which were constructed by homologous recombination of mutated TK gene of vaccinia virus with TK gene of A. foetidus were screened by adding BUdR in agar plates. Conditions for screen of TK+ A. foetidus strain, transformation of A. foetidus and selection of transformed TK- A. foetidus have been studied. By using this system, several transformed A. foetidus which contained HBsAg gene derived bf a promoter H8 cloned from genomic DNA of A. foetidus were isolated. It was demonstrated that HBsAg gene was integrated into the chromosome DNA of A. foetidus by Southern blot after many passages of spores. ELISA showed that HBsAg was positive in the growth medium (p/n = 20). The 22 nm particles which were very similar to the HBsAg particles in human serum were found in the growth medium by immunoelectromicroscope. Western blot also gave the specific bands. All these data showed that HBsAg gene was expressed in A. foetidus and the products were secreted into the growth medium. The selection system using TK gene as marker could generally be used to study the expression of foreign gene in A. foetidus.     

中国冠盘藻属的研究

本文基于近年冠盘藻属研究的新成果,对产于中国的七个种和变型作分类学及形态学、产地、生境、分布的记述。对冠盘藻属研究中的一些问题提出讨论意见。     

几种毒品简介

１９９０年１２月２８日由第七届全国人民代表大会常务委员会第十七次会议通过了《全国人大常委会关于禁毒的决定》，《决定》第一条明确了毒品的概念和范围。“毒品是指鸦片、海洛因、吗啡、大麻、可卡因、冰以及国务院规定管制的其他能够使人形成瘾癖的麻醉药品和精神药...     

浅析数字监控系统

近些年,人们对数字监控技术的需求量越来越大,被广泛的应用于生活与生产的各个领域。数字监控系统主要由摄像头、监控现场主机与远程端主机三部分组成,与模拟系统相比,它具有系统配置简单,录像方式多样,检索方式灵活,可以方便的进行警报系统的设置,访问也会有清楚的记录。本文深入分析了数字监控系统的特点以及关键技术。