建立以TK基因为标记将外源基因导入臭曲霉（Asper—gillus...

A new system for selection of transformed Aspergillus foetidus was reported. In this system, TK- A. foetidus which were constructed by homologous recombination of mutated TK gene of vaccinia virus with TK gene of A. foetidus were screened by adding BUdR in agar plates. Conditions for screen of TK+ A. foetidus strain, transformation of A. foetidus and selection of transformed TK- A. foetidus have been studied. By using this system, several transformed A. foetidus which contained HBsAg gene derived bf a promoter H8 cloned from genomic DNA of A. foetidus were isolated. It was demonstrated that HBsAg gene was integrated into the chromosome DNA of A. foetidus by Southern blot after many passages of spores. ELISA showed that HBsAg was positive in the growth medium (p/n = 20). The 22 nm particles which were very similar to the HBsAg particles in human serum were found in the growth medium by immunoelectromicroscope. Western blot also gave the specific bands. All these data showed that HBsAg gene was expressed in A. foetidus and the products were secreted into the growth medium. The selection system using TK gene as marker could generally be used to study the expression of foreign gene in A. foetidus.     

弥散神经内分泌系统

概括地介绍了弥散神经内分泌系统的组成，结构和产物，发挥作用的方式。还阐明了神经系统和胃肠胰内分泌之间可能存在某种内在联系，心血管与血细胞具有重要的内分泌功能。指出某些疾病和肿瘤与弥散神经内分泌系统有重要关系。     

“顶端优势”原理在生产实践中的应用与思考

<正>在新课标人教版高中生物必修3第三章《植物的激素调节》中,以顶端优势为例,说明植物激素之一——生长素对植物生长有两重性——既能促进生长,又能抑制生长。典型体现在植物的"顶端优势"上。顶芽产生生长素由于极性运输从而造成侧芽生长素积累,结果导致顶芽生长快,侧芽生长受到抑制。这在农业生产上对一些农作     

苹果坐果的激素调控

经红富士苹果为试材，花朵数为５的花序，其坐果率明显高于花朵数为４的花序。开花前，花朵数为５的花序，其花朵子房内ＧＡｓ、Ｚ浓度明显高于后者；开花后，后者ＧＡｓ仍然低于前者，但Ｚ浓度、Ｚ／ＧＡｓ比值迅速增加并明显高于前者。     

《Molecular Plant》文章中文摘要

2011年第4卷第4期激素作用与互作专刊（http：//mplant.oxfordjournals.org/content/vol4/issue4/index.dtl）1Joshi-Saha A, Valon C, Leung J （2011）. Abscisic acid signal off the STARTing Block. Mol Plant, 4 （4）： 562~580题目：：脱落酸信号关闭了＂起跑器＂（综述）     

蛋白质组学及其在植物营养学研究中的应用

概述了作为后基因组学时代功能基因组学研究的重要组成部分的蛋白质组学及其技术在植物营养学中的应用,包括养分和环境胁迫下植物的蛋白质组学、植物激素蛋白质组学的研究进展,并对其前景作了展望.