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Özlem Biçen Ünlüer Arzu Ersöz Ridvan Say Özlem Tomsuk Hülya Sivas 《Biotechnology progress》2013,29(2):472-479
This study describes preparation and use of novel labeled and antibodious polymeric nanolabels (anti‐alpha fetoprotein cross‐linked nanolabels) as an immunogenic and semisynthetic nanolabel with potential prognostic and therapeutic roles for hepatoma cancer. Specificity, uptake, and binding efficiencies of the nanolabel have been examined in a human hepatosarcoma cell line HepG2, a human colorectal cell line DLD‐1, and a mouse myoblast cell line C2. Labeling of the cells has been performed by treating live and fixed cells with varying concentrations of the nanolabels and then, the cells have been examined under a fluorescence microscope. In addition, all cell lines have also been labeled using FITC‐conjugated nanotrastuzumab to compare the results obtained with those of the binding of the FITC‐nanoanti‐alpha fetoprotein nanolabels. Results show that FITC‐conjugated anti‐alpha fetoprotein cross‐linked nanolabels have been taken up by both live and fixed cells and have efficiently and specifically labeled HepG2 cells at a quite low concentration. Taken all together, the results indicate that the novel targeted nanoimaging tools and technique demonstrated their ability to detect the distribution of the nanolabels as probes in hepatoma cells. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29: 472–479, 2013 相似文献
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Multilayer gold surface-enhanced Raman scattering (SERS) substrates, which consist of continuous gold films that are separated
by self-assembled monolayers (SAMs) and cast over 430-nm diameter silica nanospheres on a glass slide, have been evaluated
as a means of further enhancing the SERS signals produced from conventional metal film over nanostructure substrates. Evaluation
of the effect of various SAMs, with different terminal functional groups, on the SERS enhancement factor were measured and
compared to conventional single-layer gold film over nanostructure substrates, revealing relative enhancements as great as
22.4-fold in the case of 2-mercapto-ethanol spacer layers. In addition to evaluation of the effect of different terminal functionalities,
the effect of spacer length was also investigated, revealing that the shorter chain length alcohols provided the greatest
signals. Employing the optimal SERS multilayer geometry, SERS nanoimaging probes were fabricated and the SERS enhancement
factor and variability in enhancement factor were measured over the SERS active imaging area, providing absolute enhancements
similar to previous silver-based SERS nanoimaging probes (i.e., 1.2 × 108). Varying the size of the multilayer gold islands that were deposited on the tip of the SERS active nanoimaging probe, it
is possible to tune the optimal SERS excitation wavelength accurately and predictably over the range of approximately 450
to 600 nm, without coating the entire surface of the probe and significantly reducing the transmission and resulting signal-to-noise
ratio of the images obtained. 相似文献
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