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排序方式: 共有450条查询结果,搜索用时 93 毫秒
1.
J.G. Roddick 《Phytochemistry》1974,13(1):9-25
The literature relating to chemical, biochemical and biological aspects of the steroidal glycoalkaloid, α-tomatine, is reviewed. The alkaloid, which can be used as a starting compound for the synthesis of steroidal hormones, is toxic to a wide range of living organisms. The significance of tomatine to plants which elaborate it is discussed and some possible uses of the compound are mentioned. 相似文献
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The distribution of excreted flavonoid aglycones within the familyLabiatae was studied and differences were found, especially in the A-ring substitution patterns. Thus, 5,7-dihydroxy-6-methoxyflavones with substituted B-rings are characteristic of species ofSalvia (sect.Salvia),Rosmarinus andOcimum; 5,7-dihydroxy-6,8-dimethoxyflavones occur only inOcimum and 5,6-dihydroxy-7,8-dimethoxyflavones inThymus and related species. Members of the two subfamiliesLamioideae andNepetoideae produce exudate flavonoids, but some genera are devoid of these compounds. There is a correlation between the habitat where the plant grows and production of these compounds, the species from (semi-)arid habitats being those which generally accumulate external flavonoids. 相似文献
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In this paper, using both immunofluorescence and protein biochemistry techniques, we present definitive evidence that plasma proteins such as albumin are present within normal human epidermis. This result confirms several previous reports supporting the idea that relatively large molecules can diffuse through the epidermal basement membrane into epidermis. Our results bring new insights for discussing how hydrophobic ligands or drugs present in the bloodstream and bound to plasmatic carriers can reach epidermal cells of all layers.Abbreviations CHAPS
3-[3-cholamidopropyl dimethylammonio] propane sulfonate
- kD
kilodaltons
- BSA
bovine serum albumin
- 2ME
2-mercaptoethanol
- DTT
dithiothreitol
- SDS
sodium dodecyl sulfate
- pI
isoelectric point
- Mw
molecular weight
- Tris
Tris-(hydroxymethyl) aminomethane
- 1D
one dimensional
- 2D
two dimensional
- PAGE
poly acrylamide gel electrophoresis
- MEM
Minimal Eagle's Medium 相似文献
7.
David B. Sattelle Sarah C. R. Lummis James F. H. Wong James J. Rauh 《Neurochemical research》1991,16(3):363-374
A GABA-operated Cl– channel that is bicuculline-insensitive is abundant in the nervous tissue of cockroach, in housefly head preparations and thorax/abdomen preparations, and in similar preparations from several insect species. Bicuculline-insensitive GABA-operated Cl– channels, which are rare in vertebrates, possess sites of action of benzodiazepines, steroids and insecticides that are pharmacologically-distinct from corresponding sites on vertebrate GABAA receptors. The pharmacological profile of the benzodiazepine-binding site linked to an insect CNS GABA-operated Cl– channel resembles more closely that of vertebrate peripheral benzodiazepine-binding sites. Six pregnane steroids and certain polychlorocycloalkane insecticides, which are active att-butylbicy-clophosphorothionate (TBPS)-binding sites, also differ in their effectiveness on vertebrate and insect GABA receptors. Radioligand binding and physiological studies indicate that in insects there may be subtypes of the GABA receptor. Molecular biology offers experimental approaches to understanding the basis of this diversity.Special issue dedicated to Dr. Eugene Roberts 相似文献
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James A. Dvorak 《Journal of cellular biochemistry》1984,24(4):357-371
Trypanosoma cruzi is a heterogeneous group of parasites. The imposition of natural or artificial pressures can result in the selection of subsets of the population with concomitant changes in characteristics used to evaluate the group. In order to ascertain the extent of heterogeneity, stocks of single-cell clones were prepared from various sources. Selected cell biological, biochemical, immunochemical, parasitological, and histopathological parameters of these clones have been studied. A ten-fold difference in the rate of growth of the epimastigote stage of T cruzi clones has been observed. The extracellular growth rates of the clones correlate with the rate of growth of the obligate intracellular amastigote stage and consequently, the length of intracellular cycle of the parasite. A 40% difference in the amount of total DNA/parasite has been found between clones. Although the amount of DNA/kinetoplast and nucleus varies between clones, the major contribution to the differences in total DNA/parasite appears to be the nucleus. From 16 to 35 antigens have been demonstrated in the T cruzi clones assayed to date. Five to seven of these antigens are common to all of the stocks assayed. However, both isolate- and clone-specific antigens have also been demonstrated. The susceptibility of inbred strains of mice to T cruzi clones varies with the clone of the parasite. These data imply that the genetics of the parasite as well as the host modulate both the course and outcome of a T cruzi infection. The influence of monosaccharides on the receptor-mediated infection of vertebrate cells by trypomastigotes of T cruzi also varies between clones. The implications of these findings upon our concept and understanding of present and future problems in Chagas disease are discussed. 相似文献
10.
From the mycelium of Penicillium cyclopium a biologically active fraction (P-factor) was isolated, which increases conidiation and the formation of the benzodiazepine alkaloids cyclopenin and cyclopenol. Its activity was determined by measuring the increase of alkaloid formation in strain SM 72. On a preparative scale P-factor preparations were obtained from fermenter-grown hyphae of mutant dev 63 by extraction with water at 120°. P-factor is strongly hydrophilic but it is not a protein. It was active if added during conidiospore germination and early growth phase, causing an acceleration of protein biosynthesis. The action on alkaloid biosynthesis and sporulation is indirect and resembles that of a developmental hormone. 相似文献