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1.
Using a blood cell separator, lymphocytes were collected from otherwise healthy convalescents suffering from herpetic infections.
A specific anti-herpes dialysate (AH-DLE) was prepared from the lymphocytes, using standard procedures. Patients with recurrent
herpetic infections were treated with a single dose of the dialysate, at the initial signs of herpetic infection (group A),
with two doses (group B) or with three doses (group C). A total number of 37 patients (29 women, 8 men, age range 15–73 years)
were treated. No improvement was observed in 7 patients (18.9%), whilst 7 patients did not manifest any exacerbation of their
herpetic infection in the course of the one-year follow-up. The remaining 62.2% of the patients showed a marked improvement:
decrease of the frequency and/or duration or relapses. Before AH-DLE administration, the mean number of herpes relapses in
this group of patients was 12 p.a.. After therapy, the number of relapses decreased to 3.5 p.a.. No statistically significant
difference was observed between groups A and B. The least favourable results were registered in group C. However, this group
included 6 female patients extremely resistant to the previously therapeutic attempts, including inosiplex, non-specific DLE
or acyclovir. Thus, even in this group, the therapy was successful in 50% of the patients. 相似文献
2.
单纯疱疹病毒(HSV)Ⅰ型及Ⅱ型之间有很多共同抗原,能引起血清学交叉反应,鉴别诊断比较困难。本实验利用重组DNA技术,将部分HSV-2DNA的PstI片段克隆到载体质粒PSK中,并筛选出两个重组质粒(P和P)只与HSV-2反应,与HSV-1不反应,这两个重组质粒中所含的HSV-2DNA片段大小分别是3.1和4.3kb,另外,还筛选了一个重组质粒(PHSV2-1,含5.8kbHSV-2DNA片段)与HSV-1和HSV-2均反应。将4.3kb的片段用光生物素标记后作为探针检测了159份人阴道拭子,其中23份样品呈阳性反应,其余均为阴性,从23份阳性样品中挑选12价涂片用间接荧光抗体法检测也都呈阳性反应,随机挑选的几份杂交反应阴性样品在间接荧光试验中也是阴性。本实验制备的HSV通用及HSV-2型特异性探针将比常规的血清学方法诊断和鉴别HSV-1和HSV-2感染更为可靠。 相似文献
3.
用DNA聚合酶链反应检测单纯疱疹病毒特异性核酸,调查100名孕产妇生殖器官脂HSV感染和羊水受HSV污染的情况,结果表明:受感染的母体通过病毒护散途径及通过产道途径,污染羊水的机率分别为11.1%和54.5%;原发性HSV、继发性HSV垂直污染羊水机率分别为66.7%和29.4%;不同妊娠期和生育胎次对污染率无显著性影响。 相似文献
4.
Thyroglobulin structure-function: the effect of iodination on the structure of human thyroglobulin 总被引:1,自引:0,他引:1
Thyroglobulin of very low iodine content has been prepared from a single non-toxic human goitre. The initial iodine content of the protein (0.038%) has been increased to levels of 0.16% and 0.85% by in vitro treatment with thyroid peroxidase and the resulting proteins studied with respect to their intrinsic fluorescence, circular dichroism spectra and binding of the hydrophobic probe 1,8-anilinonaphthalene sulfonic acid (ANS). While significant differences were observed between levels of iodination in both the ANS binding and intrinsic fluorescence of the thyroglobulin, no significant differences in the near and far UV circular dichroism spectra of the protein as a function of iodine content were observed. These data suggest that, the iodination of thyroglobulin effects specific areas of the protein without significant disruption of its overall secondary structure. 相似文献
5.
Thymidine kinase activity was found in whole cell extracts of growing and stationary mouse embryo fibroblast cells after infection with murine cytomegalovirus. Determination of the kinetic constants and heat stability characteristics indicated that the enzyme activity from infected cells was different to that found in uninfected cells in the growth phase. The expression of thymidine kinase activity during virus replication was reflected by the incorporation of (6-3H) thymidine into acid precipitable fractions of infected cell cultures. Preliminary data from kinetic studies showed a reduction in the phosphorylation of thymidine by this enzyme activity in the presence of Acyclovir, a potent inhibitor of herpes virus replication. 相似文献
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8.
Background
Chondroitin sulfate proteoglycans (CSPGs) are principal pericellular and extracellular components that form regulatory milieu involving numerous biological and pathophysiological phenomena. Diverse functions of CSPGs can be mainly attributed to structural variability of their polysaccharide moieties, chondroitin sulfate glycosaminoglycans (CS-GAG). Comprehensive understanding of the regulatory mechanisms for CS biosynthesis and its catabolic processes is required in order to understand those functions.Scope of review
Here, we focus on recent advances in the study of enzymatic regulatory pathways for CS biosynthesis including successive modification/degradation, distinct CS functions, and disease phenotypes that have been revealed by perturbation of the respective enzymes in vitro and in vivo.Major conclusions
Fine-tuned machineries for CS production/degradation are crucial for the functional expression of CS chains in developmental and pathophysiological processes.General significance
Control of enzymes responsible for CS biosynthesis/catabolism is a potential target for therapeutic intervention for the CS-associated disorders. 相似文献9.
10.
Gene therapy using tissue-specific replication competent HSV 总被引:1,自引:0,他引:1
Miyatake S 《Human cell》2002,15(3):130-137