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1.
Spontaneous rupture of the Achilles tendon is increasingly common in the middle aged population. However, the cause for the particularly high incidence of injury in this age group is not well understood. Therefore, the objective of this study was to identify age-specific differences in the Achilles tendon-muscle complex using an animal model. Functional measures were performed in vivo and tissues were harvested following euthanasia for mechanical, structural, and histological analysis from young, middle aged, and old rats. Numerous alterations in tendon properties were detected across age groups, including inferior material properties (maximum stress, modulus) with increasing age. Differences in function were also observed, as older animals exhibited increased ankle joint passive stiffness and decreased propulsion force during locomotion. Macroscale differences in tendon organization were not observed, although cell density and nuclear shape did vary between age groups. Muscle fiber size and type distribution were not notably affected by age, indicating that other factors may be more responsible for age-specific Achilles tendon rupture rates. This study improves our understanding of the role of aging in Achilles tendon biomechanics and ankle function, and helps provide a potential explanation for the disparate incidence of Achilles tendon ruptures in varying age groups. 相似文献
2.
Turgor pressure and water transport properties of suspension-cultured cells of Chenopodium rubrum L.
The turgor pressure and water relation parameters were determined in single photoautotrophically grown suspension cells and in individual cells of intact leaves of Chenopodium rubrum using the miniaturized pressure probe. The stationary turgor pressure in suspension-cultured cells was in the range of betwen 3 and 5 bar. From the turgor pressure relaxation process, induced either hydrostatically (by means of the pressure probe) or osmotically, the halftime of water exchange was estimated to be 20±10 s. No polarity was observed for both ex- and endosmotic water flow. The volumetric elastic modulus, , determined from measurements of turgor pressure changes, and the corresponding changes in the fractional cell volume was determined to be in the range of between 20 and 50 bar. increases with increasing turgor pressure as observed for other higher plant and algal cells. The hydraulic conductivity, Lp, is calculated to be about 0,5–2·10–6 cm s–1 bar–1. Similar results were obtained for individual leaf cells of Ch. rubrum. Suspension cells immobilized in a cross-linked matrix of alginate (6 to 8% w/w) revealed the same values for the half-time of water exchange and for the hydraulic conductivity, Lp, provided that the turgor pressure relaxation process was generated hydrostatically by means of the pressure probe. Thus, it can be concluded that the unstirred layer from the immobilized matrix has no effect on the calculation of Lp from the turgor pressure relaxation process, using the water transport equation derived for a single cell surrounded by a large external volume. By analogy, this also holds true for Lp-values derived from turgor pressure changes generated by the pressure probe in a single cell within the leaf tissue. The fair similarity between the Lp-values measured in mesophyll cells in situ and mesophyll-like suspension cells suggests that the water transport relations of a cell within a leaf are not fundamentally different from those measured in a single cell. 相似文献
3.
The deep posterior cervical muscles (DPCM), specifically the semispinalis cervicis and cervical multifidus, are often impaired in patients with neck disorders and have been assessed by several imaging techniques. Prior ultrasound shear wave elastography (SWE) imaging and reliability assessments of the DPCM were performed utilizing similar positioning as assessments for the more superficial cervical extensors. Our objectives were to describe an SWE imaging technique for the DPCM, establish intra-rater reliability of DPCM SWE, and compare DPCM shear modulus during rest and submaximal contraction in both prone and seated positions in individuals without spinal pain. In sixteen participants, the DPCM was located using B‐mode ultrasound, then muscle shear modulus was assessed via SWE at both rest and with contraction against a 2‐kg resistance applied at the C2 spinous process. Within‐day intra‐rater reliability was moderate to good (ICC = 0.70–0.88). The DPCM were stiffer during contraction than at rest in the prone position (p = 0.002), and at rest in sitting versus at rest in prone (p = 0.003). Further research is needed to assess DPCM-specific SWE in symptomatic individuals and compare DPCM shear modulus to electromyography across contraction intensities. 相似文献
4.
Sylvie Wendling Patrick Cañadas Patrick Chabrand 《Computer methods in biomechanics and biomedical engineering》2013,16(1):45-52
The control of many cell functions including growth, migration and mechanotransduction, depends crucially on stress-induced mechanical changes in cell shape and cytoskeleton (CSK) structure. Quantitative studies have been carried out on 6-bar tensegrity models to analyse several mechanical parameters involved in the mechanical responses of adherent cells (i.e. strain hardening, internal stress and scale effects). In the present study, we attempt to generalize some characteristic mechanical laws governing spherical tensegrity structures, with a view of evaluating the mechanical behaviour of the hierarchical multi-modular CSK-structure. The numerical results obtained by studying four different tensegrity models are presented in terms of power laws and point to the existence of unique and constant relationships between the overall structural stiffness and the local properties (length, number and internal stress) of the constitutive components. 相似文献
5.
Maria Christina Dzul Stephen James Dinsmore Michael Carl Quist Daniel Bailey Gaines Kevin Patrick Wilson Michael Roy Bower Philip Michael Dixon 《Population Ecology》2013,55(2):325-341
The Devils Hole pupfish, Cyprinodon diabolis, is a federally-endangered fish that is endemic to Devils Hole, a discontiguous part of Death Valley National Park in Nye County, Nevada. Due to its status, Devils Hole pupfish monitoring must be non-obtrusive and thereby exclude techniques that require handling fish. Due to a recent decline in pupfish abundance, Devils Hole pupfish managers have expressed a need for a model that describes population dynamics. This population model would be used to identify vulnerable life history stage(s) and inform management actions. We constructed a set of individual-based simulation models designed to explore effects of population processes and evaluate assumptions. We developed a baseline model, whose output best resembled both observed length-frequency data and predicted intra-annual abundance patterns. We then ran simulations with 5 % increases in egg-larval, juvenile, and adult survival rates to better understand Devils Hole pupfish life history, thereby helping identify vulnerable life history stages that should become the target of management actions. Simulation models with temporally constant adult, juvenile, and egg-larval survival rates were able to reproduce observed length-frequency distributions and predicted intra-annual population patterns. In particular, models with monthly adult and juvenile survival rates of 80 % and an egg-larval survival rate of 4.7 % replicated patterns in observed data. Population growth was most affected by 5 % increases in egg-larval survival, whereas adult and juvenile survival rates had similar but lesser effects on population growth. Outputs from the model were used to assess factors suspected of influencing Devils Hole pupfish population decline. 相似文献
6.
For long-lived iteroparous vertebrates that annually produce few young, life history theory predicts that reproductive output
(R) and juvenile survival should influence temporal variation in population growth rate (λ) more than adult survival does. We
examined this general prediction using 15 years of mark–recapture data from a population of California spotted owls (Strix occidentalis occidentalis). We found that survival of individuals ≥1 year old (ϕ) exhibited much less temporal variability
, where CV is coefficient of variation, than R
and that R was strongly influenced by environmental stochasticity. Although λ was most sensitive (
; log-transformed sensitivity) to ϕ
and much less sensitive to either R
or juvenile survival (survival rate of owls from fledging to 1 year old;
), we estimated that R contributed as much as ϕ to the observed annual variability in λ. The contribution of juvenile survival to variability in
λ was proportional to its
These results are consistent with the hypothesis that natural selection may have favored the evolution of longevity in spotted
owls as a strategy to increase the probability of experiencing favorable years for reproduction. Our finding that annual weather
patterns that most affected R (temperature and precipitation during incubation) and ϕ (conditions during winter related to the Southern Oscillation Index)
were equally good at explaining temporal variability in λ supports the conclusion that R and ϕ were equally responsible for variability in λ. Although currently accepted conservation measures for spotted owl populations
attempt to enhance survival, our results indicated that conservation measures that target R may be as successful, as long as actions do not reduce ϕ. 相似文献
7.
Bone materials are characterized by an astonishing variability and diversity. Still, because of 'architectural constraints' due to once chosen material constituents and their physical interaction, the fundamental hierarchical organization or basic building plans of bone materials remain largely unchanged during biological evolution. Such universal patterns of microstructural organization govern the mechanical interaction of the elementary components of bone (hydroxyapatite, collagen, water; with directly measurable tissue-independent elastic properties), which are here quantified through a multiscale homogenization scheme delivering effective elastic properties of bone materials: at a scale of 10nm, long cylindrical collagen molecules, attached to each other at their ends by approximately 1.5nm long crosslinks and hosting intermolecular water inbetween, form a contiguous matrix called wet collagen. At a scale of several hundred nanometers, wet collagen and mineral crystal agglomerations interpenetrate each other, forming the mineralized fibril. At a scale of 5-10microm, the extracellular solid bone matrix is represented as collagen fibril inclusions embedded in a foam of largely disordered (extrafibrillar) mineral crystals. At a scale above the ultrastructure, where lacunae are embedded in extracellular bone matrix, the extravascular bone material is observed. Model estimates predicted from tissue-specific composition data gained from a multitude of chemical and physical tests agree remarkably well with corresponding acoustic stiffness experiments across a variety of cortical and trabecular, extracellular and extravascular materials. Besides from reconciling the well-documented, seemingly opposed concepts of 'mineral-reinforced collagen matrix' and 'collagen-reinforced mineral matrix' for bone ultrastructure, this approach opens new possibilities in the exploitation of computer tomographic data for nano-to-macro mechanics of bone organs. 相似文献
8.
Populations of the introduced Heracleum mantegazzianum consist of dense central stands, which gradually give way to open stands towards the margins. To analyse whether open stands
are due to unsuitable conditions or represent the invading front for further spread, we studied life-cycle, population dynamics,
stand structure and soil conditions of open and dense stands over two transition periods. Populations decreased during the
first interval but increased after the extremely dry and warm summer of 2003 during the second interval. Open stands had shorter
generation times, lower height, smaller proportions of small individuals and were less in equilibrium with the environment
than dense stands. In open stands, growth to higher stages was most important, while in dense stands delayed development (self-loops)
had a strong effect on population growth; stasis and fecundity contributed most to the difference in λ between stand types.
By petiole extension H. mantegazzianum may raise its leaves just above the resident vegetation. Therefore, younger stages develop faster in open stands, whereas
strong competition by conspecific adults leads to longer generation times and a higher proportion of small individuals in
dense stands. Disturbance due to extreme climatic conditions in summer 2003 equalised population dynamics of both stand types.
Life-cycle variation between stand types makes it difficult to infer simple management rules. However, our data suggest that
small and/or open stands of H. mantegazzianum may eventually serve as initials for further spread after land-use changes, whereas dense stands are stable and may represent
sources of propagules. 相似文献
9.
The surface of hydrated cells of Staphylococcus epidermidis has been probed using an atomic force microscope. While local force measurements over the surface of bacteria reveal a heterogeneous chemical surface, with heterogeneous mechanical properties, different kinds of force curves appear with high frequency, and are thought to provide information on features contributing strongly to the overall mechanical and surface behaviour of the cell. Force curves often present two different mechanical regimes, being the first one (outer) of about 48 nm thick, and presenting a local relative elasticity of about 0.08 N/m, which is about a third of the relative elasticity of the inner part of the cell wall, harder, with a relative elasticity of about 0.24 N/m, in water. Both regimes appears as straight lines in the force versus distance curves (the ‘corresponding’ stress–strain curves in contact mechanics), but hysteresis is observed between the approach and the retraction line in the inner regime, indicating a degree of viscoelasticity. No viscoelasticity is observed in the outer regime, however, which presents quite linear and juxtaposed approach-retraction lines. These kinds of force curves do not present measurable pull-off forces nor snap-in forces, which indicates an almost null interaction between tip and bacterial surface, which could be in agreement with the measured very high hydrophobicity of this strain. Another kind of force curve has been observed recurrently, showing peaks in the retraction curves. Adhesive pull-off forces were measured giving an average of about 2 nN. Interestingly, however, these force curves appear only when quite irregular and wavy retraction curves are present, from the very beginning of its trace (maximum indentation). This leads us to think that these pull-off forces measured by our AFM do not give information on surface forces-unbinding events at the surface of the bacteria, but could be related to events at the sub-surface of the cell surface. Oscillations seen in the retraction curve in the portion corresponding to the contact with the bacteria surface could be due to rupture phenomena within the multilayered cell wall architecture expected in Gram-positive bacteria as Staphylococcus epidermidis, which could result in local irreversible deformations of the cell surface. Imaging with a sharp tip in contact mode sometimes leads to surface damage. Force curves recorded over damaged parts of the cell surface showed a completely different behaviour, in many cases with two well-defined high-adhesion peaks, and also interestingly, with snap-in forces of about 0–2 nN, which seems to indicate a completely different electrical/hydrophobicity state only a few nanometers down from the surface. Similar indentation effects can occur in the contact of a bacterial cell with a solid surface, even when showing only atomic-molecular-scale roughness, thus interacting not only with the very surface of the cell, especially when soft layers are present in the outer. Our results highlight the importance of the cell surface mechanical properties and their interplay with purely surface properties when analyzing cell–material interaction, and show the AFM as a useful method for investigating this. 相似文献
10.
Natalie L. Hyslop Dirk J. Stevenson John N. Macey Lawrence D. Carlile Chris L. Jenkins Jeffrey A. Hostetler Madan K. Oli 《Population Ecology》2012,54(1):145-156
Demographic data provide a basis for understanding the life history and ecology of species, factors which are vital for informing
conservation efforts; however, little is known regarding the population ecology of most snake species, including the threatened
Eastern Indigo Snake (Drymarchon couperi). We used 11 years (1999–2009) of capture-mark-recapture (CMR) and 2.5 years (2003–2005) of radiotelemetry data from southeastern
Georgia, USA, in a CMR modeling framework to estimate apparent survival, capture and transition probabilities, and evaluate
factors influencing these parameters. The model-averaged estimate of overall apparent annual survival probability was 0.700
(±0.030 SE) and is comparable to that obtained from known fate analysis (radiotelemetry) at the same site. Body size positively
influenced survival, regardless of sex. Capture probability differed seasonally by sex, suggesting lower capture probability
for females in fall and males in winter. There was no evidence for effect of precipitation or site-specific differences in
survival. Model averaged estimate of annual adult survival estimated using multistate CMR models was 0.738 ± 0.030 and 0.515 ± 0.189
for subadults. We estimated population growth rate (λ) and elasticity (proportional sensitivity) of λ to vital rates using a stage-structured matrix population model. Population growth rate ranged from 0.96 to 1.03 depending
on the value of the probability of transitioning from subadult to adult stage. The λ was proportionally most sensitive to changes in adult survival rate, followed by subadult survival. Our results suggest that
protecting adult snakes and their habitats would result in the highest likelihood of long-term population stability and growth. 相似文献