蒲公英抗肿瘤活性部位的GC-MS分析研究

目的：考察蒲公英提取物对MCF-7增殖作用的影响,研究蒲公英抗肿瘤活性部位的化学成分。方法：采用MTT法比较蒲公英水提物、醇提物和不同极性提取物对MCF-7增殖的影响;以气相色谱．质谱（GC-MS）联用技术对具有抑制MCF-7人乳腺癌细胞增殖的部位进行分离和鉴定。结果：蒲公英水提物和醇提物对MCF-7细胞均有抑制作用,体外最大抑制率分别达到92．95％、93．59％,蒲公英不同极性溶剂提取物中,氯仿部位抑制MCF-7的增殖,且抑制作用高于醇提和水提物,乙酸乙酯和石油醚部位促进MCF-7的增殖。从蒲公英氯仿提取物中鉴定出80种化合物,占其总量的98．07％。结论：蒲公英氯仿部位具有抗肿瘤活性,为蒲公英抗肿瘤活性成分的研究提供了依据,拓展了筛选中药活性成分的方法。     

长春蒲公英雄性不育株形态学观察与败育细胞学研究

在长春蒲公英(Taraxacum junpeianum Kitam.)株群中发现雄性不育现象,为研究其败育机理及特点,探寻其不育基因,采用形态观察法、石蜡切片技术和染色体压片法,对长春蒲公英野生型及其雄性不育株的花药发育过程和花粉母细胞减数分裂过程进行了观察。结果表明:(1)长春蒲公英雄性不育株花药中部发红、干瘪、无花粉散出。与野生型比较,雄性不育株雄蕊更短,子房更窄,种子形态更加狭长;(2)长春蒲公英雄性不育株败育时期为四分体到单核小孢子前期,败育方式为小孢子自身异常发育,绒毡层异常分解,互相粘连败育;(3)长春蒲公英雄性不育株花粉母细胞减数分裂二分体时期出现落后微核,随后产生极少四分体,并且四分体产生大量染色体桥,小孢子营养物质流失,彻底败育。因此,长春蒲公英雄性不育株败育彻底、稳定,并且有种的特点。小孢子自身异常发育和绒毡层异常分解是导致败育的主要原因。     

Oxalic Acid Production and Mycelial Biomass Yield of Sclerotinia minor for the Formulation Enhancement of a Granular Turf Bioherbicide

The fungus Sclerotinia minor is presently under development in this laboratory as a granular bioherbicide for broadleaf weed species. With a view to enhancing the virulence of the fungus, the effect of increasing endogenous oxalic acid concentration through modification of the growth conditions was investigated. S. minor was grown in 125 ml of eight different liquid culture media in shake flasks incubated at 20°C for 7 days. The final pH, mycelial dry weight, and oxalic acid content of the spent growth media were determined and the virulence of S. minor grown on each solid culture medium was screened on detached dandelion leaves. A 330% increase in oxalic acid was obtained plus 56 mM of sodium succinate to Modified Richard's solution (MRS) as compared to MRS alone. A concomitant increase in virulence of 218% was expressed as increased lesion diameter.     

"蒲公英-蝌蚪-鳝鱼"食物链的物流与能流分析

本文对食物链“蒲公英—蝌蚪—鳝鱼”的物质流和能量流作了定量分析。结果表明：该食物链经济产品的物质、能量转化效率较低,生物量转化效率为1.1％,能量转化效率为0．8％,N、P转化效率分别为3．1％、1．5％。但该食物链刺激鳝鱼食欲,有利于鳝鱼繁殖。     

Land-use selection by dandelions in the Tokyo metropolitan area,Japan

Land use by native and introduced dandelion groups in man-made habitats was studied at 7090 survey points in the Tokyo metropolitan area, Japan, in order to clarify the replacement phenomenon occurring among dandelions. In the center of Tokyo, where the native group was rare, the percentage occurrence of the introduced group was lower than in other regions, whereas in the outskirts of Tokyo, both groups showed much higher occurrence. Roadsides, vacant areas and urban land-use categories were significantly favorable for growth of the introduced group, whereas preserved or rural land use was more favorable for the native group. Seed fertility in the introduced group was more than 80%, and was independent of population size, whereas in the native group it was not as high, and for a small population was dependent on population size, being stable at about 70% in populations larger than 70 plants. Thus, both large populations and solitary plants in the introduced group appeared to act as seed sources, making it likely that the introduced group will spread increasingly through roadsides and urban land, which are becoming more widespread as a result of urbanization. It is feared that, unless deliberately retained, the amount of preserved or rural land which has fostered large populations of the native group will further decrease with urbanization and other forms of development, thus depleting the seed sources and reducing the population.     

Influence of substrate concentration and bicarbonate addition on the anaerobic digestion of an inulin-rich substrate as the ending stage in a dandelion biorefinery scheme

This study was focused on the suitability of dandelion plant as part of a biorefinery scheme in which anaerobic degradation (biomethanization) for biogas production is the ending process. Inulin as the main compound in the tuberous root was selected as substrate and tested at different concentrations (5, 10 and 15 g TS L⁻¹). The presence of bicarbonate as a buffer on anaerobic digestion was assessed as well. Methane production curves were fitted with the modified Gompertz equation. Methane production yield (Y_CH4), specific maximum methane production rate (SR_MAX) and the first-order hydrolysis constant (k_H) were affected by the substrate concentration. Bicarbonate was found not to be determinant for the overall biomethanization process because no significant effect over the Y_CH4was achieved in the presence or absence of bicarbonate; between 0.19 to 0.23 m³ kg^-1 VS added. However, k_Hand SR_MAX were affected by the presence of this inorganic compound. Without the addition of bicarbonate, k_Hand SR_MAX decreased as the TS increased. These results will be key for a dandelion biorefinery proof-of-concept under the proposed conditions.     

Some Mathematical Relationships of Biological Value

Several ideas are given for using material from dandelion plants for class practical work. One purpose of the paper is to show how readily available material can sometimes substitute for the more commonly recommended one, for the purposes of demonstration and investigation. It is hoped that a more imaginative approach by teachers, in choosing experimental material for classes, might broaden students' views of biology and help to eliminate the concept of the ’type’.     

Insect herbivory and plant adaptation in an early successional community*

To address the role of insect herbivores in adaptation of plant populations and the persistence of selection through succession, we manipulated herbivory in a long‐term field experiment. We suppressed insects in half of 16 plots over nine years and examined the genotypic structure and chemical defense of common dandelion (Taraxacum officinale), a naturally colonizing perennial apomictic plant. Insect suppression doubled dandelion abundance in the first few years, but had negligible effects thereafter. Using microsatellite DNA markers, we genotyped >2500 plants and demonstrate that insect suppression altered the genotypic composition of plots in both sampling years. Phenotypic and genotypic estimates of defensive terpenes and phenolics from the field plots allowed us to infer phenotypic plasticity and the response of dandelion populations to insect‐mediated natural selection. The effects of insect suppression on plant chemistry were, indeed, driven both by plasticity and plant genotypic identity. In particular, di‐phenolic inositol esters were more abundant in plots exposed to herbivory (due to the genotypic composition of the plots) and were also induced in response to herbivory. This field experiment thus demonstrates evolutionary sorting of plant genotypes in response to insect herbivores that was in same direction as the plastic defensive response within genotypes.     

HPLC analysis of geometrical isomers of lutein epoxide isolated from dandelion (Taraxacum officinale F. Weber ex Wiggers)

Lutein epoxide has been isolated from petals of dandelion (Taraxacum officinale F. Weber ex Wiggers) by thin-layer chromatography (TLC) on silica to be used for the accurate identification of this carotenoid in other sources. The extract was analyzed by high-performance liquid chromatography (HPLC) using a C(30) column, as a result of which six geometrical isomers were separated. The identification of these isomers was performed on the basis of their UV/vis spectroscopic features in the mobile phase. In quantitative terms, it was observed that all-E-lutein epoxide was the major carotenoid and that there were also high amounts of the (9Z)- and (9'Z)-isomers, although the latter may be an artifact.     

Constitutive knox1 gene expression in dandelion (Taraxacum officinale, Web.) changes leaf morphology from simple to compound

Seed plants with compound leaves constitute a polyphyletic group, but studies of diverse taxa show that genes of the class 1 KNOTTED-LIKE HOMEOBOX (KNOX1) family are often involved in compound leaf development. This suggests that knox1 genes have been recruited on multiple occasions during angiosperm evolution (Bharathan et al. in Science 296:1858–1860, 2002). In agreement with this, we demonstrate that the simple leaf of dandelion (Taraxacum officinale Web.) can be converted into a compound leaf by the constitutive expression of heterologous knox1 genes. Dandelion is a rosette plant of the family Asteraceae, characterised by simple leaves with deeply lobed margins and endogenous knox1 gene expression. Transgenic dandelion plants constitutively expressing the barley (Hordeum vulgare L.) hooded gene (bkn3, barley knox3) or the related bkn1 gene, developed compound leaves featuring epiphyllous rosettes. We discuss these results in the context of two current models of compound leaf formation.Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users.