Sustainability of SARS-CoV-2 Induced Humoral Immune Responses in COVID-19 Patients from Hospitalization to Convalescence Over Six Months

Virologica Sinica - Understanding the persistence of antibody in convalescent COVID-19 patients may help to answer the current major concerns such as the risk of reinfection, the protection period...     

Neutralization of five SARS-CoV-2 variants of concern by convalescent and BBIBP-CorV vaccinee serum

The prevalence of SARS-CoV-2 variants of concern (VOCs) is still escalating throughout the world. However, the level of neutralization of the inactivated viral vaccine recipients’ sera and convalescent sera against all VOCs, including B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron) remains to be lack of comparative analysis. Therefore, we constructed pseudoviruses of five VOCs using a lentiviral-based system and analyzed their viral infectivity and neutralization resistance to convalescent and BBIBP-CorV vaccinee serum at different times. Our results show that, compared with the wild-type strain (WT), five VOC pseudoviruses showed higher infection, of which B.1.617.2 and B.1.1.529 variant pseudoviruses exhibited higher infection rates than wild-type or other VOC strains, respectively. Sera from 10 vaccinated individuals at the 1, 3 and 5-month post second dose or from 10 convalescent at 14 and 200 days after discharge retained neutralizing activity against all strains but exhibited decreased neutralization activity significantly against the five VOC variant pseudoviruses over time compared to WT. Notably, 100% (30/30) of the vaccinee serum samples showed more than a 2.5-fold reduction in neutralizing activity against B.1.1.529, and 90% (18/20) of the convalescent serum samples showed more than 2.5-fold reduction in neutralization against B.1.1.529. These findings demonstrate the reduced protection against the VOCs in vaccinated and convalescent individuals over time, indicating that it is necessary to have a booster shot and develop new vaccines capable of eliciting broad neutralization antibodies.     

通督调神针法联合穴位贴敷对老年气虚血瘀证缺血性脑卒中恢复期患者脑血流、血液流变学和血清NGF、BDNF的影响

摘要 目的：探讨通督调神针法联合穴位贴敷对老年气虚血瘀证缺血性脑卒中恢复期患者脑血流、血液流变学和血清神经生长因子（NGF）、脑源性神经营养因子（BDNF）的影响。方法：本次研究纳入我院于2021年3月~2022年5月期间收治的70例老年气虚血瘀证缺血性脑卒中恢复期患者。采用信封抽签法分为对照组（n=35）和观察组（n=35）。对照组接受常规治疗,观察组在对照组的基础上接受通督调神针法联合穴位贴敷治疗。对比两组疗效、美国国立卫生研究院卒中量表（NIHSS）评分、中医证候积分、脑卒中专用生活质量量表（SS-QOL）评分、Fugl-Meyer运动功能量表（FMA）评分、脑血流指标、血液流变学指标和NGF、BDNF水平。结果：观察组的临床总有效率为94.29%（33/35）,高于对照组的71.43%（25/35）（P<0.05）。观察组治疗4周后SS-QOL评分、FMA评分高于对照组同期,中医证候总积分、NIHSS评分低于对照组同期（P<0.05）。观察组治疗4周后血流阻力指数（RI）、搏动指数（PI）低于对照组同期,平均血流速度（Vm）高于对照组同期（P<0.05）。观察组治疗4周后全血低切黏度、血浆比黏度、全血高切黏度、纤维蛋白原均低于对照组同期（P<0.05）。观察组治疗4周后血清NGF、BDNF水平均高于对照组同期（P<0.05）。结论：通督调神针法联合穴位贴敷可有效改善老年气虚血瘀证缺血性脑卒中恢复期患者的脑血流、血液流变学,调节血清NGF、BDNF水平。     

Patients with Prolonged Positivity of SARS-CoV-2 RNA Benefit from Convalescent Plasma Therapy: A Retrospective Study

Virologica Sinica - Convalescent plasma therapy has been implemented in a few cases of severe coronavirus disease 2019. No report about convalescent plasma therapy in treating patients with...